血清磷酸化高分子量神经微丝蛋白与大鼠脊髓损伤程度的相关性

Correlation between Serum Phosphorylated High- molecular- weight Neurofilament and Severity of Spinal Cord Injury in Rats

目的研究血清磷酸化高分子量神经微丝蛋白(p NF-H)在脊髓损伤动物模型中的动态变化规律及其与脊髓损伤程度的相关性。方法 20只Sprague-Dawley大鼠等分为空白对照(A)组及脊髓损伤轻(B)、中(C)、重(D)度组,检测大鼠血清p NF-H水平、BBB评分及残存白质面积百分比。结果 B、C、D组大鼠血清p NF-H在术后12 h及3 d出现两个高峰;3组间有显著性差异(P<0.05);损伤后3 d血清p NF-H水平与14 d时BBB评分、残存白质面积百分比呈负相关性(r=-0.987,r=-0.978)。结论脊髓损伤后大鼠血清p NF-H水平存在双峰现象;脊髓损伤程度越重,大鼠p NF-H水平越高;p NF-H水平可以预测脊髓损伤严重程度。

Objective To investigate the level of phosphorylated high-molecular-weight neurofilament（p NF-H） after spinal cord injury（SCI）, and explore the relationship between p NF-H and severity of SCI. Methods 20 Sprague-Dawley rats were equally divided into control group（group A）, mild SCI group（group B）, moderate SCI group（group C） and severe SCI group（group D）. The level of p NF-H in serum,BBB score and remaining area of white matter were obtained at different time points. Results The level of serum p NF-H in groups B, C and D arrived at peaks 12 hours and 3 days after SCI, and there was significant difference among them（P〈0.05）. Both BBB score and remaining area of white matter 14 days after SCI negatively correlated with the level of p NF-H 3 days after SCI（r=-0.987 and r=-0.978, respectively）. Conclusion The p NF-H increases twice after SCI in rats, and may be associated with the severe of SCI, which can be considered as a biomarker.