甘丙肽2型受体激动剂对慢性温和应激模型大鼠抑郁样行为的影响

Effects of Galanin Receptor 2 Agonist on Depression-like Behavior in Chronic Mild Stress Model Rats

目的侧脑室注射甘丙肽2型受体(Gal R2)激动剂AR-M1896,探讨其对慢性温和应激模型(CMS)大鼠抑郁样行为的影响。方法 48只Sprague-Dawley大鼠随机等分为对照组、CMS组、CMS人工脑脊液(a CSF)组、CMS AR-M1896组。对照组不作任何处理,其他3组制备大鼠慢性温和应激模型。应激6周后,通过强迫游泳及糖水偏好实验验证抑郁样行为;挑选造模成功大鼠,CMSaCSF组和CMS AR-M1896组侧脑室分别注射a CSF和AR-M1896。观察强迫游泳实验中的不动时间、攀爬时间,以及糖水偏好实验中的糖水饮用百分比。结果与CMSaCSF组相比,CMS AR-M1896组不动时间明显缩短(F=11.998,P<0.01),攀爬时间延长(F=8.268,P<0.05),糖水饮用百分比明显增加(F=10.352,P<0.01)。结论侧脑室注射AR-M1896可对慢性温和应激模型大鼠产生抗抑郁作用。

Objective To explore the effect of intracerebroventricular injection of AR-M1896, a galanin receptor 2 agonist, on depression- like behavior in rat chronic mild stress（CMS） model. Methods 48 Sprague- Dawley rats were randomly assigned into control group,CMS group, CMS artificial cerebrospinal fluid（a CSF） group and CMS AR-M1896 group equally. The control group received no intervention, and the other groups were established chronic mild stress model. After six-week of stress, forced swim test and sucrose preference test were conducted to identify the CMS rats. AR-M1896 oraCSF was injected into the lateral ventricle of CMS AR-M1896 group and CMSaCSF group, respectively. The immobility time and climbing time in the forced swim test were analysed, and the sucrose consumption percentage in the sucrose preference test was measured. Results The immobility time decreased（F=11.998, P〈0.01）, climbing time increased（F=8.268, P〈0.05）, and the sucrose consumption percentage increased（F=10.352, P〈0.01） in CMS AR-M1896 group, compared with CMSaCSF group. Conclusion Intracerebroventricular administration of galanin receptor 2 agonist AR- M1896 is effective on depression in CMS model rats.