非离子型微乳液增敏荷移反应光度法测定饲料中三聚氰胺

Determination of Melamine in Animal Feed via Spectrophotometry by Sensitized Reaction of Non-Ionic Microemulsion with Charge Transfer

OP/异戊醇/甲苯/水非离子型微乳液对电子给体三聚氰胺与π电子受体茜素红的荷移反应有增敏作用,通过实验确定了微乳液增敏荷移反应的分光光度法测定条件。实验结果表明,微乳液增敏三聚氰胺与茜素红生成荷移配合物的最大吸收波长为560 nm,配合物配位比1∶1.3,茜素红用量1.0 m L,温度55℃,加热反应时间40 min,p H 7.0为最佳反应条件,检出限为42.26 mg/L。

OP / isoamylol / methylbenzene / H2 O microemulsion had strong sensitivity on the color reactions of the charge transfer reaction between melamine as electron donor and alizarin red as π-electron acceptor. The results showed thatmelamine could react with alizarin red to form an 1∶ 1. 3 complex under the condition of microemulsion sensitization. The maximum absorption peak was560 nm. The reagent dosage of alizarin red 1. 0 m L,temperature 55 ℃,heating reaction time 40 min and p H 7. 0 were the optimum reaction conditions. The detection limit was 42. 26 mg / L.