氨基酸铜配合物的抗氧化活性分析

Amino Acid Copper Complexes Antioxidant Activity Analysis

采用氨基酸和醋酸铜为原料,设计合成4种不同配体结构的Cu（Ⅱ）金属配合物Cu L1~4,并用紫外分光光度计和傅里叶红外检测仪对其化学结构进行了分析。采用改进的氮蓝四唑还原法确定了氨基酸铜金属配合物抗氧化活性最强时对应的浓度,并讨论了在相同浓度下不同种类氨基铜酸配合物的抗氧化活性与其化学结构之间的关系。结果表明,L-α-丙氨酸铜配合物由于金属活性中心周围空间位阻较小,其抗氧化活性高于苯丙氨酸铜配合物。

Amino acids and copper acetate were used as raw materials to synthesize four kinds of Cu（ Ⅱ） metal complexes（ Cu L1 ~ 4）,the structure of complex was confirmed by UV and IR. The anti-oxidant activities and the optimal concentration were measured by using NBT illumination method. The relationship of the antioxidant activity of different type amino acid complexes and their chemical structure at the same concentration was discussed. The result shows that L-α-alanine has highest antioxidant activity because the copper complex has less steric hindrance around the metal center.