阳离子脂质体DLin-MC3-DMA合成

Synthesis of Cationic Lipid DLin-MC3-DMA

可电离的阳离子脂质体DLin-MC3-DMA是一种高效的siRNA运输载体,它能有效封装相应的siRNA并使其进入细胞质内,然后两者分离,siRNA发挥其功效。以价格低廉的亚油酸为起始原料,经还原、溴代、格氏和缩合等4步反应,高效合成了目标化合物DLin-MC3-DMA,不仅缩短了反应步骤,而且有效降低了成本。

DLin-MC3-DMA,as an efficient transport agent of siRNA,can package the siRNA to form LNPs / siRNA complex,and then transport them into cytoplasm efficiently. Afterwards,the complex separates and the siRNA plays its function. In this work,DLin-MC3-DMA was high efficiently synthesized via four steps including reduction,bromination,Grignard reaction,and condensation with cheaper linoleic acid as the starting material. The method shortened the reaction procedure and lowered the cost effectively as well.