血府逐瘀汤促血管新生中EphB4/ephrinB2信号通路的作用研究

Roles of EphB4/ephrinB2 in Angiogenesis induced by Xuefu Zhuyu Decoction

目的:探讨Eph B4/ephrin B2信号通路在血府逐瘀汤促血管新生中的作用。方法:运用血清药理学方法,以1.25%、2.5%、5%浓度的血府逐瘀汤含药血清和空白血清干预人脐静脉内皮细胞(Human Umbilical Vein Endothelial Cells,HUVEC-12)48h,通过MTT法、Boyden小室迁移法、细胞黏附法、逆转录PCR法分别检测细胞增殖、迁移、黏附、Eph B4和Ephrin B2基因表达的情况。结果:与同血清含量的对照组比较,5%浓度含药血清对细胞的增殖能力起抑制作用。1.25%、2.5%和5%3个浓度含药血清皆有显著的促内皮细胞迁移和黏附作用,并能抑制Eph B4的表达,其中1.25%、2.5%浓度含药血清抑制Ephrin B2的表达。结论:血府逐瘀汤通过促内皮细胞迁移和黏附发挥促血管新生作用,且该作用可能与Eph B4/Ephrin B2通路密切相关。

object To explore the roles of EphB4/ephrinB2 in Angiogenesis induced by Xuefu Zhuyu Decoction （XZD）. Methods： Using serum pharmacology technique to study the cell proliferation, migration, adhesion, ephrinB2 and EphB4 gene expression by MTT, Boyden chamber, ceil adhesion and reverse transcriptase polymerase chain reaction（ RT- PCR） separately on Human Umbilical Vein Endothelial Cells （HUVEC-12） after intervened by 1.25% , 2.5% and 5% XZD containing serums for 48h. Results Comparison with the control group of same serum level , 5% medicated serum inhibited cell proliferation. 1.25% , 2.5% and 5% medicated serum had significant effect on promoting endothelial cell migration or adhesion and inhibition the EphB4 expression. 1.25% and 2.5% medicated serum inhibited EphrinB2 expression. Conclusion XZD had significant effect on cell migration and adhesion to promote angiogencsis which is closely related to the EphB4/ EphrinB2.