用于糖链抗原CA19-9实时动态活检的免疫双光子荧光标记试剂盒

Two-photon Fluorescent Labeling Kit for the Real-time Dynamic Biopsy of CA19-9

采用双光子荧光标记探针LP制备了免疫双光子荧光抗体LP-Ab,以特异性识别肿瘤标志物CA19-9(Ag)形成抗原-抗体复合物(LP-Ab·Ag),基于双光子诱导荧光机制用近红外激光(740 nm)激发LP-Ab·Ag,利用LP-Ab·Ag的双光子荧光实现了CA19-9的高清晰度、高灵敏度及高分辨率的量化检测和实时动态成像,并制成用于糖链抗原CA19-9检测的简单便捷的双光子荧光标记试剂盒.

Carbohydrate antigen CA19-9（ Ag） is a tumor marker associated with pancreatic cancer,gallbladder cancer,colon cancer and gastric cancer in early stage of tumor and cancer stage. Antibody Ab19-9（ Ab）to carbohydrate antigens were labeled to form probe-labeled antibody LP-Ab using the prepared two-photon fluorescence probe LP. On the basis of the antigen-antibody specificity affinity principle,LP-Ab could combine with carbohydrate antigen Ag into a complex LP-Ab·Ag,which was excited by near infrared laser（ 740nm） and emitted strong two-photon fluorescence to accomplish the high sensitive biopsy and real-time dynamic high-resolution imaging of the tumor marker Ag with two-photon fluorescence microscope. Based on this,a simple and convenient two-photon fluorescent labeling kit was prepared to realize the rapid detection of tumor,which is convenient for large-scale clinic testing and screening. The research further broadens the application of LP,and provides a sharp tool for revealing the mechanism of cancer occurrence,development and metastasis. LP-Ab can be used to observe and study the dynamic transfer mechanisms of the tumor markers in cells and the mechanism of carcinogenesis.