用于检测细胞内谷胱甘肽的比率型荧光探针

Ratiometric Fluorescent Probe for the Detection of Glutathione in Living Cells

设计合成了1种用于检测生物巯基的比率型荧光探针（4）,并考察了其对谷胱甘肽的识别作用.在4-羟乙基哌嗪乙磺酸（HEPES）缓冲液中,探针4可与谷胱甘肽快速反应,溶液颜色由淡黄色变为粉红色,从而实现＂裸眼＂检测,且在608 nm处的荧光信号增强.在1.6×10^（-5）～2×10^（-4）mol/L范围内,探针4能够定量检测谷胱甘肽,检出限为8.9×10^（-7）mol/L.此外,探针4还可用于MCF-7细胞中谷胱甘肽的成像.

A fluorescent probe（ 4） for the detection of biothiols was designed and synthesized and its properties for labeling glutathione was investigated. After reaction with glutathione in 2-[4-（ 2-hydroxyethyl）-1-piperazinyl]ethanesulfonic acid（ HEPES） buffer,the color of solution changed from light yellow to pink,which could be detected by naked eyes. Meanwhile,the fluorescence was enhanced at 608 nm. Probe 4 was useful for measuring glutathione at concentrations ranging from 1. 6 ×10^（-5）to 2 ×10^（-4）mol / L with a detection limit of 8. 9 ×10^（- 7）mol / L. Besides,probe 4 was sensitive to glutathione in MCF-7 cells,which emitted red fluorescence when it was incubated with MCF-7 cells.