生物兼容蛋白水凝胶微图案的光刻平版印刷

Preparation of Biocompatible Protein-hydrogel-based Micro-patterns via UV Lithography

通过简易的紫外光刻平版印刷技术,对无化学修饰的生物兼容性良好的天然牛白蛋白的水相光刻胶进行微图案化加工,获得了可用作微光栅器件的蛋白质微条纹结构,表征了蛋白质凝胶微条纹图案的宏观性状.结果表明,其折射散射彩虹色明显,单束激光经过后的衍射条纹对称度高,可获得11级衍射条纹.通过改变参数实现了条纹的可控褶皱.将图案化的蛋白水凝胶膜用于肝癌细胞的培养,实现了细胞图案化排布.

Through facile UV lithography,without chemical modification,biocompatible natural proteinhydrogel-based film and micro-patterns were prepared. The micro-patterns can be used as micro-grating optical devices. Then rainbow feature was observed and diffraction fringe for a 638 nm laser beam was obtained under white light,even with a diffraction level up to 11. Further more,micro-wrinkles could be controlled by changing related parameters. Finally,Hep G-2 cells were cultured on the protein micro-patterns and cell micro-patterns were obtained.