调脂保肝汤对非酒精性脂肪肝模型大鼠肝功能、血脂及血糖代谢影响的实验研究

Experimental study of Tiaozhi-baogan decoction on liver function and metabolism of glucose and lipid in rats with non-alcoholic fatty liver disease

目的观察中药调脂保肝汤对非酒精性脂肪肝(NAFLD)模型大鼠肝功能、血脂及血糖代谢的影响,探讨其治疗NAFLD的作用机制。方法将44只Wistar大鼠随机分为空白组12只和模型组32只,空白组予普通饲料喂养,模型组予高脂饲料喂养。以随机选取2只大鼠达到预期NAFLD病理组织学时间为准,造模8周。造模成功后,模型组大鼠再随机分为高剂量组、低剂量组及对照组,每组10只。高剂量组予相当于生药0.15 g/m L的调脂保肝汤浓缩中药液灌胃,低剂量组予相当于生药0.075 g/m L的调脂保肝汤浓缩中药液灌胃,对照组予等容积0.9%氯化钠注射液灌胃。8周后计算4组大鼠的肝指数,观察4组大鼠肝功能指标[谷氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)及γ-谷氨酰转移酶(γ-GT)]、血脂指标[甘油三酯(TG)及总胆固醇(TC)]及空腹血糖(FPG)水平。结果对照组大鼠肝指数明显高于空白组(P<0.05),高、低剂量组大鼠较对照组均可降低肝指数(P<0.05),且高、低剂量组组间比较差异无统计学意义(P>0.05);对照组大鼠ALT、AST及γ-GT水平均高于空白组(P<0.05),高、低剂量组大鼠较对照组均可降低ALT及AST水平(P<0.05),且高、低剂量组组间比较差异无统计学意义(P>0.05);高剂量组大鼠较对照组可降低γ-GT水平(P<0.05),而低剂量组γ-GT水平与对照组比较差异无统计学意义(P>0.05);对照组大鼠TC、TG及FPG水平均高于空白组(P<0.05),高、低剂量组较对照组均可降低TC、TG及FPG水平(P<0.05),高剂量组较低剂量组降低效果更明显(P<0.05)。结论调脂保肝汤对NAFLD疗效确切,其作用机制可能与减少肝细胞脂肪沉积、改善肝功能及纠正血糖、血脂代谢紊乱有关。

Objective To observe the effects of Tiaozhi-baogan decoction on liver function and metabolism of glucose and lipid in rats with non-alcoholic fatty liver disease（ NAFLD） and its mechanism of action.Methods44 Wistar rats were randomly divided into blank group（ n = 12） and model group（ n = 32）.Rats in blank group were fed with general diet,and rats in model group were fed with high-fat diet for 8 weeks.After successful modeling,rats in model group were randomly divided into high dose,low dose and control group,10 rats in each group.High dose group were received concentrate liquid of Chinese herbal medicine（ crude drug 0.15 g /m L） by gavage,low dose group were received concentrate liquid of Chinese herbal medicine（ crude drug 0.075 g /m L） by gavage and control group were received 0.9% sodium chloride injection by gavage.The liver index was calculated in four groups after 8 weeks.The liver functions including alanine transaminase（ ALT）,aspartate aminotransferase（ AST） and gamma glutamyl transferase（ γ-GT）,the blood lipid parameters including triglyceride（ TG） and total cholesterol（ TC）,and fasting blood-glucose（ FPG） were observed in four groups.Results The liver index in control groupwas obviously higher than that in blank group（ P〈0.05）.The liver indexes in high and low dose group were decreased as compared with those in control group（ P〈0.05）,and there was no statistical difference between high and low dose group（ P〈0.05）.The levels of ALT,AST and γ-GT incontrol group were higher than those in blank group（ P〈0.05）,and those in high and low dose group was lower than those in control group（ P〈0.05）,and there was no statistical difference between high and low dose group（ P〉0.05）.The level of γ-GT in high dose group was lower than that in control group（ P〈0.05）,and there was no statistical difference between low dose group and control group（ P〉0.05）.The levels of TC,TG and FPG were higher than those in blank group（ P〈0.05）,and those in high and low dose group were decreased as compared with those in control group（ P〈0.05）,the decrease in high dose group was more obvious than that in low dose group（ P〈0.05）.Conclusion Tiaozhi-baogan decoction has exact effect on NAFLD,its mechanism may be related with the decrease of fat deposition in liver cell,the improvement of liver function,and rectify the derangement in blood glucose and lipid.