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酿酒酵母组蛋白H3K14乙酰化对H3K4三甲基化的影响 被引量：1

The Effect of Histone H3K14 Acetylation on H3K4 Tri-methylation in Saccharomyces cerevisiae
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摘要 首先构建酿酒酵母BY4741组蛋白H3第14位赖氨酸突变菌株,该位点突变后则不能被乙酰化.然后通过Western blot检测突变菌株和未突变菌株(对照菌株)的H3K4三甲基化.结果表明,H3K14突变菌株中未检测到H3K4三甲基化,而作为对照的未突变菌株能够检验到H3K4三甲基化修饰.该结果显示酿酒酵母组蛋白H3K14乙酰化能够对H3K4三甲基化产生影响. Firstly,the mutant strains at H3 lysine 14 of S. cerevisiae BY4741 was constructed. The site could not be acetylated after mutant. Then H3K4 tri-methylations of mutant strains and non-mutant strains（ control strains） were detected by Western blot. The results showed that H3K4 tri-methylations were not found in mutant strains at H3 lysine14,while H3K4 tri-methylations in non-mutant strainsused as control were detected. It suggests that histone H3K14 acetylation can affect H3K4 tri-methylation in S. cerevisiae.
作者 史晨霞 薛涛涛 赵秀娟 周媛 梁栋 崔向军
机构地区 内蒙古科技大学数理与生物工程学院 内蒙古科技大学生物工程与技术研究所
出处 《信阳师范学院学报（自然科学版）》 CAS 北大核心 2016年第2期200-203,共4页 Journal of Xinyang Normal University(Natural Science Edition)
基金 国家自然科学基金项目(31260274 61361014) 内蒙古自然科学基金项目(2015MS0334)
关键词 组蛋白修饰 甲基化 乙酰化 因果关系 histone modification methylation acetylation causative relationship
分类号 Q75 [生物学—分子生物学]
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参考文献13

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二级参考文献36

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信阳师范学院学报（自然科学版）
2016年 第2期

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