盐酸小檗碱对草鱼补体系统及补体c3作用的研究

The effects of berberine hydrochloride on complement system and complement c3 in Ctenopharyngodon idellus

为了探究盐酸小檗碱(berberine hydrochloride)对草鱼(Ctenopharyngodon idellus)补体c3的作用机理,用含0、0.06%、0.12%、0.24%盐酸小檗碱饲料投喂草鱼,在第7 d、14 d检测血清中补体c3含量,在第14 d检测肝脏中补体c3 mRNA表达量及急性感染嗜水气单胞菌(Aeromonas hydrophila)后存活情况。并进一步对盐酸小檗碱体内药代动力学和补体消耗试验进行了研究。结果显示;摄食盐酸小檗碱的草鱼血清中补体c3含量和肝脏补体c3 mRNA表达量均显著性增加(P<0.05/P<0.05),草鱼的存活率与对照组相比也有显著性提高(P<0.01)。补体消耗试验结果显示,当盐酸小檗碱在血清中浓度小于5 mg/L时与对照组相比补体消耗不显著,当盐酸小檗碱浓度大于5 mg/L时,补体消耗达到显著性差异(P<0.05),表明此时盐酸小檗碱与补体c3直接结合,激活补体。药代动力学实验发现,灌服盐酸小檗碱后,出现双峰现象,两峰值分别是0.243 mg/L和0.117 mg/L,此浓度远远低于与补体c3直接结合量最低量(5 mg/L)。结果表明,盐酸小檗碱对草鱼补体c3的作用机理可能是通过上调补体c3mRNA的表达增加补体c3数量,而非通过直接结合补体c3来改变其活性。

To explore the effect of berberine hydrochloride on the complement c3 of Ctenopharyngodon idellus,the level of complement c3 in serum were studied for 7 day and 14 day,the mRNA expression of c3 in the liver and resistance against acute Aeromonas hydrophila infection in C. idellus were evaluated after 14 days of feeding with berberine hydrochloride at the rate of 0,0. 06%,0. 12%,0. 24%,respectively. The pharmacokinetics in vivo and complement consumption test in vitro of berberine hydrochloride were further studied. The results showed that the complement c3 levels in serum and the mRNA expression of c3 in the liver of fish fed with berberine hydrochloride increased significantly when compared with the control（ P〈0. 05 / P〈0. 01）. The survival rate was significantly higher in groups fed with berberine hydrochloride than the control（ P〈0. 01）. In addition,complement consumption showed significant difference only when the concentration of berberine hydrochloride was higher than 5 mg / L. Berberine hydrochloride combined with complement molecules directly and the complement system was activated at this time. The pharmacokinetics experiment found that bimodal phenomena appearedafter taking berberine hydrochloride and the value of two peaks was 0. 243 mg / L and 0. 117 mg / L,respectively,which was much lower than 5 mg / L. The results suggested that the berberine hydrochloride could enhance the immunity of C. idellus and the effect of berberine hydrochloride on the complement c3 in C. idellus might not be by combining with complement molecules directly,but through up- regulating the mRNA expression of c3 to increase the quantity of c3 protein.