摘要
利用传统PCR和ABI 3730测序技术对暗纹东方鲀(Takifugu obscurus)Amhr2基因外显子9进行了SNP7271检测和测序分析,并对该SNP位点与暗纹东方鲀性别相关性进行了研究。结果显示,在SNP7271位点发生了G/C碱基突变,产生了CG、CC两种基因型。SNP7271和暗纹东方鲀性别存在显著相关性(P<0.01)。基因型为CG的个体生理性别为雄性,基因型CC的个体生理性别为雌性。
In order to identify gender of Takifugu obscurus before reproduction season and save the input of aquaculture of T. obscurus,SNP7271 of exton 9 of T. obscus Amhr2 gene were detected by PCR and DNA sequencing in present study.The correlation analysis between the polymorphism of Amhr2 and sex determination was studied. The results indicated that a G / C mutation at the position SNP7271 of the Amhr2 gene by sequencing and resulted in 2 genotype（ GG,GC）. There was significant correlation between SNP7271 and the gender of T. obscurus. The genotype CG was male and the genotype CC was female.
出处
《淡水渔业》
CSCD
北大核心
2016年第2期99-102,共4页
Freshwater Fisheries
基金
中国水产科学研究院淡水渔业研究中心中央级公益性科研院所基本科研业务费专项资金项目(2013JBFR07)
江苏省水产三新工程项目(D2014-9)
