广西不同产地广山楂中总黄酮含量测定

Content Determination of Total Flavonoids in Malus doumeri (Bois) chev.by Visual Spectrophotometry from Different Producing Area in Guangxi Province

目的：建立广山楂总黄酮含量的测定方法,比较广西不同产地广山楂中总黄酮的含量。方法：采用可见分光光度法,以芦丁为对照品,采用硝酸铝显色反应,在505nm处测定广山楂总黄酮含量。结果：芦丁浓度在0.016 4-0.065 6mg/mL范围内线性关系良好,r=0.999 5,平均回收率为98.53%（RSD=1.39%）。不同产地广山楂总黄酮含量存在差异,其中广西贺州产广山楂总黄酮含量最高。结论：该方法简便、准确、重现性好,可用于广山楂的质量控制。

Objective：To establish a method for determining the total flavonoids in Malus doumeri（Bois）chev.from different producing area in Guangxi province by visual spectrophotometry.Methods：The content of total flavonoids was detected by visual spectrophotometry.Rutin was performed as a control and the sample with sodium nitrite-aluminum nitrate and sodium hydroxide as color-developing agent.The content of total flavonoids from Malus doumeri（Bois）chev.was determined at 505nm.Results：The good linearity between concentrations and absorbance of rutin was found within the range of 0.016 4-0.065 6（mg/mL）（r=0.999 5）.The average recovery was 98.53%,and the RSD was 1.39%.According to the results from visual data,the content of rutin of Malus doumeri（Bois）chev.was various from different producing area.The highest content of rutin was found in Malus doumeri（Bois）chev.produced in Hezhou.Conclusion：This method can be used for determining flavonoids in Malus doumeri（Bois）chev.for its rapidness,simplicity,accurateness andreliability.