摘要
利用产油微生物合成含量丰富的油脂,对于解决石化能源日益紧缺问题,改善人类生活水平具有重要意义。金属离子能影响产油微生物生长形态、细胞内外渗透压和油脂合成关键酶活力等,对产油微生物油脂合成有重要的调控作用。本文首先介绍了产油微生物的产油机制,随后重点阐述了金属离子对产油微生物油脂积累的影响及其分子机理,最后对进一步探讨金属离子在产油微生物发酵过程中的作用研究提出一些建议。文章指出由于产油微生物油脂合成途径不尽相同,在工业上利用产油微生物生产油脂时,应从该微生物油脂合成的主要途径入手,找出该途径中的关键酶,随后充分考虑不同微生物对金属离子的耐受性、不同金属离子对微生物形态和胞内关键酶活力的影响,以及不同金属离子之间对同种关键酶的活性中心是否存在竞争或协同的关系等,从而制定可行的金属离子添加控制策略。
Using oleaginous microorganism to produce lipid is meaningful for overcoming fossil-fuel shortage and improving human life quality. Metal ions can affect the growth morphology,intra-/extra-cellular osmotic pressure and the activity of the key enzyme during the oleaginous microorganism lipid accumulation. Therefore,metal ions are important regulators for oleaginous microorganism. In this review,we discussed the mechanism of oleaginous microorganism lipid accumulation and then explained which metal ions affected oleaginous microorganism and how. Finally,we gave some suggestions on the future studies in exploring the role of metal ions in oleaginous microorganism fermentation process. In order to develop feasible metal ion addition and control strategy for oleaginous microorganism,the following factors might need to be considered. First, we should find the key enzyme from various lipid synthesis pathway of oleaginous microorganism. Then we should fully consider the metal ion tolerance of the target oleaginous microorganism,the effects of different metal ion on the microorganism morphology and the activities of the key intracellular enzymes. Moreover,we should explore the competitive or collaborative relationship among different metal ions when these metal ions could all affect the active centers of the key enzymes.
出处
《化工进展》
EI
CAS
CSCD
北大核心
2016年第4期1173-1179,共7页
Chemical Industry and Engineering Progress
基金
国家自然科学基金(21506096,21476111)
国家高技术研究发展计划(2014AA021703)
江苏省自然科学基金(BK20131405)
农业部农产品加工重点实验室开放课题(2015011)
江苏高校优势学科建设工程项目
关键词
金属离子
酶
产油微生物
代谢
发酵
多不饱和脂肪酸
油脂
metal ions
enzyme
oleaginous microorganism
metabolism
fermentation
polyunsaturated fatty acids
lipid