抗除草剂bar基因与EPSPS基因在转基因甘蔗中的应用研究

On Application of Herbicide Resistant Gene bar and EPSPS in Transgenic Sugarcane

通过农杆菌介导法,分别将bar基因和EPSPS基因导入到甘蔗中,分别获得了抗草铵膦和草甘膦两种除草剂的转基因甘蔗。通过直接喷洒田间施用浓度的草铵膦和草甘膦除草剂,证明了转基因甘蔗T0、T1代、宿根1代和宿根2代外源基因遗传稳定,且均具有稳定的耐除草剂的功能。因此转耐除草剂基因的甘蔗,可以与转耐除草剂基因的玉米、大豆、油菜和棉花一样,有广阔的应用前景。

In this research gene bar and EPSPS were transferred into sugarcane cultivar via agrobacterium-mediated method,and the transgenic sugarcane lines resistant to glufosinate-ammonium and glyphosphate were obtained. Directly spraying two herbicides at application concentration for field onto the field planting the transgenic sugarcane lines demonstrated the genetic stability of exogenous gene in generation T0,T1,ratoon 1,and 2 of them,and the lines had the stable herbicide-resistant ability. Therefore,the transgenic sugarcane lines resistant to herbicide can be widely used in the field production as the other transgenic herbicide-resistant crops such as corn,soybean,rape and cotton.