基于细菌同源蛋白预测细菌最适生长温度的研究

Prediction of Optimal Growth Temperature of Bacterium Based on the Homologous Proteins

不同细菌有不同的最适生长温度,而基因序列与其最适生长温度密切相关。为探究其相关性,选取92个具有不同最适生长温度的细菌的全基因组序列为研究材料,通过寻找92个细菌共有的同源蛋白,并计算共有同源蛋白中氨基酸的频率,发现共有同源蛋白的氨基酸频率特征与其最适生长温度存在着显著的相关关系,其中蛋白质序列中的螺旋结构与其最适生长温度关系最大。该研究为揭示细菌对温度的适应机制,以及对蛋白质稳定性相关的分子设计具有重要的意义。

The optimal temperature for each bacterium differs,which is related to its gene sequence. In order to explore the correlation between them,the known genome sequences of 92 bacteria with own different optimal temperatures were selected as the study material,then the common homologous protein from 92 bacteria were searched,and frequencies of the amino acids in homologous protein were calculated. A significant correlation between the frequency of the amino acid in homologous protein and the optimal growth temperature was realized. The analysis of the sites in homologous genes showed that the helix regions in the protein sequence were the most correlated with its optimal growth temperature. This study presents important significance on understanding the mechanism of the bacterial adaption to the temperature as well as designing the mutation to improve the protein stability.