刺槐根瘤菌与纤维素分解菌对上海青和高粱的促生效应

Combined Rhizobium of Robinia pseudoacacia with Cellulose-decomposing Bacteria for Promoting Growth of Shanghai Green( Brassica chinensis) And Sorghum vulgare

通过盆栽试验,用刺槐根瘤菌(Rhizobium of Robinia pseudoacacia)与纤维素分解菌(Cellulose-decomposing Bacteria)对高粱和上海青进行单独接种和混合接种,采用针刺、浸种和涂叶3种接种方法,测量其各种生长指标。初步探究刺槐根瘤菌与纤维素分解菌联合对禾本科作物高粱和双子叶作物上海青两种非豆科植物的促生效应。结果表明,在非针刺条件下,两种菌混接组(B组)比单独接种根瘤菌组(D组)的促生效应更显著,上海青B组在灭菌条件下的根长促生率比D组高出48.97%,高粱B组在非灭菌条件下干重的促生率比D组高出30.76%;而且除了高粱的B组干重以外,盆栽试验条件下,B、D两组的其他生长指标在灭菌情况下的促生率比非灭菌条件下的促生率高,差别最大的为上海青B组鲜重,灭菌条件下比非灭菌条件下高出47.13%。根瘤菌与纤维素分解菌混合接种非针刺组,对高粱和上海青的各项生长指标促生效应明显,可为今后进一步开发非豆科作物菌肥提供试验依据。

The growth-promoting effect of Rhizobium with cellulose-decomposing bacteria（ CDB） on Sorghum vulgare and Shanghai green（ Brassica chinensis） using pot experiment with Rhizobia and CDB single and mixed inoculation to the plants was carried out,using acupuncture,seed soaking,and leaf daubing three inoculation methods,and determine their various growth indices. The growth promotion effects of Rhizobium combined with CDB on sorghum of graminaceous crops and Shanghai green of dicotyledonous crops these two non-fabaceous crops were initially probed deeply into. The results showed that under the conditions of non-stab inoculation of combined with two kinds of bacteria（ group B） had more significant effects on their growth promotion than that inoculated with single Rhizobium（ group D）. Under sterilized conditions,the growth rate of root length of Shanghai green was over 48. 97% higher than that of group D,and the dried weight in group B of S. vulgare grew higher than 30. 76%; Moreover,the other growth promotion indices of group B and D with sterilized pot experiment,the growth promotion rate was higher than that of the nonsterilized pot experiment. And in addition,the dried weight of S. vulgare in group B,under sterilized conditions,the largest difference was the Shanghai green＇s fresh weight,under sterilized conditions it was higher by 47. 13% than that under non-sterilized conditions. The growth promoting effect of combined Rhizobium with CDB on S. vulgare and Shanghai green of non-stab groups had significant effects on various growth promotion indices,these can provide an experimental foundation for further development of microbial fertilizer for crops of non- fabaceous family in the future.