miR-338基因簇与食管癌发病风险的病例对照研究

A case-control study on the risk of esophageal carcinoma and miR-338 cluster

目的:探讨mi R-338基因簇在食管癌组织中的表达模式及其与食管癌发病风险的关系。方法:选取86例经病理确诊的食管癌患者,分别提取食管癌和癌旁组织总RNA,采用实时荧光定量PCR法检测hsa-mi R-338-3p、hsa-mi R-338-5p与hsa-mi R-657的表达,应用配对样本t检验分析癌和癌旁组织中mi RNA表达差异,Pearson相关分析检验基因簇各mi RNA表达的相关性,logistic回归分析mi RNA异常表达对食管癌发病风险的影响。结果:hsa-mi R-338-3p与hsa-mi R-338-5p在食管癌组织中的表达均较癌旁组织降低(P均<0.05),hsa-mi R-657在食管癌和癌旁组织中表达的差异无统计学意义(P>0.05),hsa-mi R-338-3p与hsa-mi R-338-5p表达显著相关(r=0.754,P<0.05),hsa-mi R-338-5p的低表达增加了食管癌的发病风险(OR=1.264,P<0.05),可能是食管癌的危险因素。结论:mi R-338基因簇可能抑制了食管癌的发生,hsa-mi R-338-5p可能成为食管癌诊断的潜在标志物。

OBJECTIVE： To investigate the expression level of miR-338 cluster in esophageal carcinoma and identify the relationship between miR-338 cluster and esophageal carcinoma. METHODS：A total of 86 cases of patients with confirmed esophageal carcinoma were selected,real time RT-PCR were performed to detect the expression of hsa-miR-338-3p,hsa-miR-338-5p and hsa-miR-657. We used paired t-test to analyze the expression of miRNA in tumor and adjacent non-tumor tissues,Pearson correlation analysis to examine the correlation of the expression of each miRNA and logistic regression a nalysis to analyze t he e ffect of a bnormal expression of miRNAs on the risk of esophageal cancer. RESULTS：hsamiR-338-3p and hsa-miR-338-5p were downregulated in tumor tissues compared with adjacent non-tumor tissues（P〈0.05）,and no statical difference was found of the expression of hsa-miR-657 between the two tissues（P〉0.05）. The expression of hsa-miR-338-3p and hsa-miR-338-5p were significantly correlated（r=0.754,P〈0.05）,and low expression of hsa-miR-338-5p was the risk factor for esophageal carcinoma（OR=1.264,P〈0.05）. CONCLUSION：miR-338 cluster may play an important role in the formation process of esophageal carcinoma. In addition hsa-miR-338-5p may represent the miR-338 cluster to be a useful biomarker for diagnosis.