棉花枯萎病菌培养条件的研究

Research of Culture Condition on Cotton Fusarium oxysporum f.sp. vasinfectum

以棉花枯萎菌为试验材料,研究不同温度、pH值对枯萎菌菌落生长速度和菌丝生长状态的影响,目的在于进一步了解枯萎菌的极限生长条件,以期明确棉花枯萎病菌生理生态特性,为棉花枯萎病的检疫检测和综合治理提供依据。结果表明,供试菌株的最适生长温度为28℃,最高极限生长温度为35℃,最低极限生长温度为10℃。在25~28℃范围内菌落生长速度无明显变化,但生长状态有变化。温度为34℃时,生长受到明显抑制。供试菌株生长的最适pH值为7~12,最高极限生长pH值为13,最低极限生长pH值为4。

In order to study the effects of different temperature and pH on the rate of mycelium growth and conidia production, Anyang Fusarium oxysporum f.sp. vasinfectumusing was used as experimental material.The purpose is to shed light on the utmost limit of growth condition of Fusarium oxysporum f.sp. vasinfectum, make clear the physiological and ecological properties of it and provide foundations for quarantine detection and comprehensive treatment. The results showed that the range of temperature was from 10 ℃ to35 ℃ for the mycelium growth of Fusarium oxysporum f.sp. vasinfectum, and the optimum temperature was 28 ℃.There was not a distinct variations on the rate of mycelium growth at the range from 25 ℃ to 28 ℃,but growth state had some variations. Growth was inhibited obviously at the temperature of 34 ℃, pH was from 4 to 13,with the optimum being 7 to 12.