气—质联用测定新疆石榴籽油中脂肪酸含量

Determination of fatty acids from Xinjiang pomegranate seed oil by gas chromatography mass spectrometry

对石榴籽油中的脂肪酸成分进行测定研究。通过氢氧化钾-甲酯酯化反应,以十一烷酸甲酯为内标物,正己烷为萃取溶剂,采用气相色谱—质谱联用技术对石榴籽油中脂肪酸甲酯成分进行分析,辅助NIST库检索并分析各组分。建立内标法测定石榴籽油中脂肪酸含量的方法。结果表明,石榴籽油中主要成分为石榴酸,其含量超过70%(w/w)。该方法前处理简便快捷,结果准确度高,分析时间较短,可为内标法测定脂肪酸含量提供借鉴。

Study on fatty acid composition of commercially available samples pomegranate see d oil. Through esterification reaction,using hexane as solvent direct injection and quantified by internal stand-ard C11∶0 methyl ester.Gas chromatography -mass spectrometry was used for analysis of fatty acid methyl ester,and NIST library search to identify various components.The results showed that punicic acid were the main fatty acids in the sample which content more than 70%(w/w).This method is simple and fast, the analytic time is short and the result is accurate,which can provide reference for the similar products of fatty acid content.