白介素17E在过敏性哮喘患者嗜酸粒细胞表达的研究

Interleukin-17 E expression on eosinophils in allergic asthmatics

目的 ：评价白介素17E（IL-17E）对过敏性哮喘患者嗜酸粒细胞的作用。方法 ：以流式细胞检测的方法检测14例过敏性哮喘患者、15例过敏性非哮喘患者、14名无过敏正常人嗜酸粒细胞IL-17E及受体的表达,以酶联免疫吸附法（ELISA）测定血浆IL-17E的水平,并比较了不同组别间的差异。结果：过敏性哮喘患者血浆IL-17E水平[145（64~290）ng/L]显著高于无过敏正常人群[21（0~116）ng/L]（P=0.012）。血浆IL-17E水平与第1秒用力呼气容积（FEV_1）占预计值百分比（FEV_1%）呈显著负相关（r=-0.443,P=0.003）。嗜酸粒细胞表面IL-17E特异性受体IL-17R B的表达在过敏性哮喘患者中[43.08%（33.96%~59.98%）]显著高于过敏性非哮喘患者[11.98%（6.33%~27.11%）]（P=0.002）和无过敏正常人群[17.70%（10.97%~38.9%）]（P=0.01）,IL-17RA的表达也在过敏性哮喘患者中[97.94%（94.25%~98.74%）]显著高于过敏性非哮喘者[86.60%（27.48%~97.64%）]（P=0.036）和无过敏正常人群[93.24%（42.38%~97.36%）]（P=0.027）。嗜酸粒细胞表面IL-17RB的表达与IL-17RA的表达相关（r=0.809,P=0.001）。过敏性哮喘患者血浆IL-17E水平为145（64~290）ng/L,显著高于无过敏正常人群[21（0~116）ng/L]（P=0.012）,但与过敏性非哮喘者[80（42~156）ng/L]比较无显著差异（P=0.275）。结论：过敏性哮喘患者血浆IL-17E水平、嗜酸粒细胞表达其受体IL-17RA和IL-17RB增高,提示IL-17E在过敏性炎症中嗜酸粒细胞的活化中发挥作用。

Objective To elucidate the effect of interleukin-17E（IL-17E） on eosinophils in allergic asthmatic patients.Methods IL-17 E, IL-17 RA and IL-17 RB expressions on eosinophils in normal subjects, atopic non-asthmatic and allergic asthmatic patients were detected by flow cytometry, and plasma level of IL-17 E was measured by enzyme linked immunosorbent assay（ELISA）. The results of these measurements were analyzed and compared. Results Plasma level of IL-17 E increased significantly in allergic asthmatics when compared with normal controls [145（64-290） ng/L vs 21（0-116）ng/L,（P =0.012）], but no significant difference was found between allergic asthmatics and atopic non-asthmatics [80（42-156） ng/L,（P=0.275）]. Plasma IL-17 E level was negatively correlated with percentage of first minute forced expiratory volume to predicted value（FEV_1%）（r=-0.443, P=0.003）. The expression of IL-17 RB on eosinophils was significantly higher in allergic asthmatics [43.08%（33.96%-59.98%）] than that in atopic non-asthmatics [11.98%（6.33%-27.11%）]（P=0.002）and normal controls [17.70%（10.97%-38.9%）]（P=0.01）. The expression of IL-17 RA was also significantly higher in allergic asthmatics [97.94%（94.25%-98.74%）] than that in atopic non-asthmatics [86.60%（27.48%-97.64%）]（P=0.036） and normal controls [93.24%（42.38%-97.36%）]（P=0.027）. The expression of IL-17 RB was positively correlated with expression of IL17RA（r=0.809, P =0.001）. Conclusions Plasma level of IL-17 E and expressions of IL-17 RA and IL-17 RB on eosinophils are increased in patients with allergic asthma, and it is suggested that IL-17 E has some effects on activation of eosinophils in allergic inflammation.