miRNA-200a调控缺血缺氧后新生大鼠海马神经干细胞的增殖

miRNA-200a regulation after ischemia hypoxia with the newborn rat hippocampal neural stem cell proliferation

目的研究miRNA-200a对缺血缺氧后新生大鼠海马神经干细胞增殖的调控作用。方法建立新生7天SD大鼠缺血缺氧性脑病(HIE)模型,利用免疫组化方法检测缺血缺氧后新生大鼠海马CA1区内源性神经干细胞的增殖情况;利用real-time PCR技术同步检测miRNA-200a的表达;分析miRNA-200a与缺血缺氧诱导的内源性神经干细胞增殖之间的关系;结果模型动物大脑行HE染色提示颈动脉结扎侧枕顶叶皮层和海马的CA1、CA3区存在明显坏死区域,提示模型动物建立成功。免疫组化结果显示Brdu阳性的神经干细胞分布于脑内各个区域,缺血缺氧组海马CA1区阳性细胞数比正常组明显增多。real-time PCR结果提示缺血缺氧组大鼠海马miRNA-200a的表达量比正常组明显增高。结论 miRNA-200a能促进缺血缺氧后新生大鼠海马神经干细胞的增殖。

Objective： To study the miRNA- 200 a of newborn rat hippocampal neural stem cells after ischemia anoxic proliferation regulation function. Methods： to establish a newborn SD rats 7 days of hypoxic ischemia encephalopathy（ HIE） model,by using immunohistochemical method to detect ischemia hypoxia newborn rat hippocampal CA1 region after the proliferation of endogenous neural stem cells; Using real- time PCR technology synchronous detection of micrornas-200 a expression; Analysis the miRNA- 200- a induced by ischemia and hypoxia of the relationship between endogenous neural stem cell proliferation,Results： the model of the animal＇s brain line HE dyeing prompt carotid artery ligation side pillow parietal cortex and hippocampus CA1,CA3 area exists obvious necrosis area,suggests to establish animal models with success. Immunohistochemical results show that the Brdu positive neural stem cells in the brain regions,ischemia hypoxia group of hippocampal CA1 area of positive cells was increased significantly than the normal group. Real- time PCR results suggest ischemia hypoxia group rats hippocampal micrornas- 200 a expression quantity is significantly higher than the normal group. Conclusion： the miRNA- 200 a can promote the blood anoxic after newborn rat hippocampal neural stem cell proliferation.