HPV E6/E7 mRNA表达量与宫颈癌患者预后的关联研究

Analysis of the relationship between HPV E6/E7 mRNA expression and prognosis of cervical carcinoma

目的研究HPV E6/E7 mRNA的表达量与宫颈癌患者预后的关系。方法实时荧光定时PCR检测宫颈癌患者癌旁正常组织和癌组织中HPV E6/E7 mRNA的表达情况;分析HPV E6/E7 mRNA的表达量与宫颈癌临床病理特征、患者预后的关系。结果宫颈癌组织中HPV E6/E7 mRNA的表达明显高于癌旁正常组织。高表达HPV E6/E7 mRNA与肿瘤大小(P=0.025)和癌旁浸润(P=0.031)呈正相关。另外,高表达HPV E6/E7 mRNA还与低总生存率(P=0.032)和无瘤生存率(P=0.02)相关。多变量分析发现,HPV E6/E7 mRNA的表达是一个独立的预后因子。结论 HPV E6/E7 mRNA的表达在宫颈癌的发生、发展中起重要作用,是患者预后的重要评价因子。

Objective： To investigate the HPV E6 / E7 mRNA expression in cervical carcinoma and to estimate its clinical significance. Methods： The expression of HPV E6 / E7 mRNA was evaluated by Real- time PCR in cervical cancer and adjacent normal cervical tissues. The correlation between HPV E6 / E7 mRNA expression and clinicopathological features were statistically analyzed. Results： HPV E6 / E7 mRNA expression was significantly higher in cervical cancer tissues compared with that in adjacent normal cervix. High HPV E6 / E7 mRNA expression was positively correlated with tumor size（ P= 0. 025） and parametrial infiltration（ P = 0. 031）. Moreover,high levels of HPV E6 / E7 mRNA was associated with lower overall survival（ P = 0. 032） and recurrent- free survival（ P = 0. 02）. Multivariate analysis showed that the expression of HPV E6 / E7 mRNA was an independent factor of recurrent- free survival for the patients with cervical carcinoma Conclusion： HPV E6 / E7 mRNA expression may play an important role in the development and progression of cervical carcinoma,and may be a potential factor for cervical carcinoma prognosis.