阻抗在细胞检测中的应用进展

The application process of impedance sensing in cell detection

细胞阻抗传感技术是细胞培养和阻抗技术的结合,将细胞培养在工作电极上,通过施加微小的电压,收集流经包括细胞在内的整个系统的电流信号来检测细胞的状态。阻抗技术由于实时、连续、不破坏性、无需标记等优点,已被广泛应用于细胞性质测定、癌细胞检测和抗癌药物筛选等方面。该文综述了阻抗技术在细胞检测中的应用和进展,包括:1、阻抗检测电极的设计;2、阻抗监测细胞生理状态;3、阻抗在细胞毒性和药物筛选中的应用;4、阻抗区分正常细胞与癌细胞。

Electric Cell-substrate Impedance Sensing is the combination of cell culture and impedance technology.Relevant cells cultured on working electrode are subjected to an alternating electric field, when cells attach and spread on this electrode, current signals of the whole system are collected to perceive cells state. With advantages like real-time, continuous, non-invasive, and label-free, impedance technology has been widely used in cell properties determination, cancer cells detection and anticancer drug screening etc. In this paper, we briefly review the recent application process of impedance in cell detection, involving the design of the detection system, the impedance monitoring of the physiological state of cells, the application of impedance in cytotoxicity and drug test,the differentiation of normal cells and cancer cells by impedance.