系统性红斑狼疮患者血清sCR1含量及临床意义

Quantitative Determination and Clinical Significance of sCR1 in Patients with Systemic Lupus Erythematosus

目的探讨可溶性补体受体1型(sCR1)在人血清中的含量与系统性红斑狼疮(SLE)免疫球蛋白(Ig)和补体(C)之间的相关性。方法采用本中心建立的检测人血清微量sCR1双抗体ELISA夹心法,检测了48例活动期、12例缓解期SLE患者和50例健康对照者血清中sCR1含量,同时测定了血清中IgG、IgA、IgM、C3、C4和抗核抗体谱(IgG)含量,并对所有SLE患者做活动性测量(SLAM)评分。结果活动期SLE患者血清中的sCR1含量为(57.68±5.67)ng·ml-1,明显高于健康对照组(32.54±3.28)ng·ml-1及缓解期SLE患者(37.0±2.93)ng·ml-1;健康人和活动期SLE患者比较有统计学差异(P<0.05)。活动期SLE患者血清中的sCR1与IgG呈正相关(P<0.05),与C3呈负相关(P<0.05),与SLAM呈线性正相关(P<0.01)。结论 SLE患者血清sCR1的含量升高和C3的降低均与SLE活动程度及病情加重有关,血清sCR1的含量对了解SLE病情和判定治疗效果具有一定的临床意义。

Objective To explore the association between the content of human complement receptor 1（ sCRI） in human plasma and immunoglobulin（ Ig）,complement（ C） in systemic lupus erythematosus（ SLE）. Methods Forty-eight active SLE patients,twelve stable SLE patients and fifty healthy controls were recruited. sCR1 in human plasma was determined by the double antibody sandwich ELISA method which we had developed. Levels of IgG,IgA,IgM,C3,C4 and ENA（ IgG） were measured,and the lupus activity index（ SLAM） of each SLE patient was evaluated. Results The levels of s CR1 of SLE patients in active group〔（ 57. 68 ± 5. 67） ng·ml^-1〕were obviously higher than those in control 〔（ 32. 54 ± 3. 28） ng·ml^-1〕and stable 〔（ 37. 0 ± 2. 93） ng·ml^-1〕group.（ P〈0. 05）. The levels of sCR1 in patients with active SLE were positively correlated with IgG（ P〈0. 05）,but negatively with C3（ P〈0. 05）,and linearly positively correlated with SLAM（ P〈0. 01）. Conclusion The level of sCR1 is evidently associated with the activity of the disease and can be regarded as a good marker of SLE activity.