杂色鲍血细胞分类、结构和免疫功能的流式细胞术分析

Classification, structure, and immune functions of abalone(Haliotis diversicolor) hemocytes using a flow cytometric analysis

应用流式细胞术对杂色鲍(Haliotis diversicolor Reeve)血细胞的分类、结构和免疫功能进行分析。根据细胞前向角散射光(FSC)和侧向角散射光(SSC)强度的不同,可将血细胞分为三个亚群:透明细胞、小颗粒细胞和大颗粒细胞,组成比例分别为32.71%、58.17%和8.55%。血细胞的平均总凋亡和死亡率为3.76%。血细胞对荧光微球的总吞噬率为63.67%,其中吞噬1个、2个、3个及以上荧光微球的血细胞分别占22.31%、16.39%、24.96%。线粒体数量、溶酶体数量、非特异性酯酶活性和非诱导性活性氧(ROS)含量均在大颗粒细胞中最高,透明细胞最低。结果表明,杂色鲍三类血细胞在结构和功能上均存在差异,两类颗粒细胞可能在鲍类免疫过程中发挥着更为重要的作用。

The classification, structure, and immune functions of abalone（Haliotis diversicolor） hemocytes were analyzed using flow cytometry. Based on the differences of forward light scatter（FSC） and side light scatter（SSC）, hemocytes could be divided into three subpopulations, hyalinocyte, small granulocyte, and large granulocyte in the proportions of 32.71%, 58.17% and 8.55%, respectively. The average apoptotic ratio in all hemocytes was 3.76%. The total phagocytic ratio of hemocytes was 63.67%, and the percentages of cells that internalized one, two, and three or more fluorescent beads were 22.31%, 16.39%, and 24.96%, respectively. Mitochondria mass, lysosome mass, non-specific esterase activity, and non-induced reactive oxygen species（ROS） production were highest in the large granulocyte and lowest in the hyalinocyte. These results indicate that structure and immune functions are different between the three hemocyte subpopulations, and that small granulocytes and large granulocytes may play a more important role in the immune response of H. diversicolor.