琼枝麒麟菜多糖抗呼吸道病毒活性研究

Anti-respirovirus activity of a Eucheuma gelatinae polysaccharide

为了评价琼枝麒麟菜多糖(EGP)包括粗提物(CEGP)及大于500 k Da多糖组分(LEGP)抗呼吸道相关病毒活性。采用Reed-muench法计算流感病毒H1N1、H3N2和H5N3,柯萨奇病毒CVB3及呼吸道合胞病毒RSV-long株滴度;采用观察细胞病变效应(CPE)法,确定CEGP和LEGP对犬肾细胞(MDCK),人宫颈癌细胞(He La)和人喉癌上皮细胞(HEp-2)的最大无毒浓度;通过细胞病变观察法(CPE)及四甲基偶氮唑盐微量酶反应比色法(MTT法)测定CEGP和LEGP抑制各病毒产生细胞病变的作用。结果表明:CEGP和LEGP对5种常见的呼吸道病毒H1N1、H3N2和H5N3,CVB3和RSV都有抑制作用,CVB3和RSV对CEGP和LEGP更敏感;CEGP和LEGP有抗多种呼吸道病毒作用,为抗呼吸道病毒新药的研发提供理论依据。

Objective： To evaluate the anti-respirovirus effects of crude Eucheuma gelatinae polysaccharides（CEGP） and large molecular weight components（MW 500 k Da, LEGP）. Method： The Reed-Muench method was used to calculate the titer of the influenza virus（H1N1, H5N3, and H3N2）, coxsackie virus（CVB3）, and the respiratory syncytial virus（RSV-long）. The maximum no toxicity concentrations of CEGP and LEGP to each cell line（MDCK, He La, and HEp-2） were selected by the cytopathic effect（CPE） method. The antiviral effects of CEGP and LEGP were evaluated by the MTT and CPE methods. Results： Both CEGP and LEGP showed significant and similar inhibition effects on the influenza virus（H1N1, H5N3, and H3N2）, CVB3, and RSV-long. CVB3 and RSV were more sensitive to EGP（CEGP and LEGP） compared with the influenza virus. Conclusion： CEGP and LEGP showed great potential in respirovirus inhibition.