采用基质辅助激光解吸/电离飞行时间质谱(MALDI-TOF)鉴定曲霉属Flavi组和Fumigati组的一些种（英文）

Identification of some Aspergillus species in the Flavi and Fumigati sections by matrix-assisted laser desorption / ionisation time-of-flight(MALDI- TOF) mass spectrometry

一些曲霉是主要的食物腐败菌及人的病原体,特别是免疫系统受损的病人可能导致严重的感染。本研究评价了利用基质辅助激光解吸电离飞行时间质谱对一些临床和环境中重要的Flavi组和Fumigati组曲霉,进行鉴定的可能性,并将结果与形态学及测序结果(ITS区和部分-tubulin和钙调蛋白基因)进行比较分析。通过曲霉中34个Flavi组菌株和30个Fumigati组菌株的质谱分析,来建立基质辅助激光解吸电离飞行时间质谱的数据库。对光谱数据进行聚类分析表明Fumigati组的基质辅助激光解吸电离飞行时间质谱的结果与系统发育结果完全一致;Flavi组的基质辅助激光解吸电离飞行时间质谱方法将A.flavus,A.oryzae,A.sojae和A.parasiticus分开的效果比测序方法好。随后,再选取用于验证数据库的50个菌株中49个(98%)菌株用质谱数据得到正确鉴定。对于分离本研究中曲霉的隐形种,基质辅助激光解吸电离飞行时间质谱方法优于测序方法。这种方法可以用于曲霉临床实验室鉴定的标准方法,因为临床需要快速和稳定的鉴定方法,这对于适当的治疗方案的选择很重要,此方法同样适于环境研究工作。

Several Aspergillus species are important food spoilage moulds and human pathogens and particularly in immunocompromised patients they may cause severe invasive infections. This study evaluated MALDI-TOF MS for the identification of some clinically and environmentally important Aspergillus taxa in the sections Flavi and Fumigati and compared the results with those obtained by morphology and sequencing（ ITS regions and partial -tubulin and calmodulin genes）.We used mass spectra from 34 strains belonging to the Aspergillus section Flavi and from 30 strains of the section Fumigati to create a MALDI-TOF MS database. Cluster analysis of the spectra showed perfect concordance of the MALDI-TOF MS results with the phylogenetic grouping of species in the Fumigati section; In the Flavi section MALDI-TOF MS separated A. flavus,A. oryzae,A. sojae and A. parasiticus better than sequencing. Of the 50 strains subsequently used to validate the database,49（ 98%） were identified correctly by mass spectrometry.MALDI-TOF MS seems to be superior to sequencing in separating the cryptic Aspergillus species investigated.The method could become a standard technique for Aspergillus identification in clinical laboratories,where a fast and reliable identification is important for adequate treatment,as well as in environmental work.