HPLC-柱后光化学衍生法检测花生酱中黄曲霉毒素

Determination of Aflatoxin B1,B2,G1 and G2 in Peanut Butter by HPLC with On-Line PostColumn Photochemical Derivatization

建立高效液相色谱-在线柱后光化学衍生-荧光检测器检测花生酱中黄曲霉毒素B1、B2、G1、G2的含量。样品以乙腈-水(80∶20)溶液提取,经免疫亲和柱净化后,利用在线柱后光化学衍生-HPLC-FLD进行分析测定。结果:在优化条件下,黄曲霉毒素B1、G1在0.30 mg/L^10 mg/L,黄曲霉毒素B2、G2在0.06 mg/L^3.0mg/L线性关系良好,r>0.998,回收率80%~101%,RSD<5.9%。黄曲霉毒素B1、B2、G1、G2的检测限(LOD)分别为0.10、0.03、0.15、0.04μg/kg。

To determine the contents of aflatoxin B1,B2,G1 and G2 in peanut butter using on-line post-column photo-chemical derivatization-HPLC-FLD method. The samples were extracted with acetonitril-H2O（80 ∶ 20）and purified with inmunoafinity column,aflatoxins were analyzed by HPLC-FLD with post-column photochemical derivatizaton. On optimum conditions,aflatoxin B1,G1 ranging 0.30 mg/L-10 mg/L showed a good linear relationship with aflatoxin B2,G2 ranging 0.06 mg/L-3.0mg/L with r0.998. The recoveries ranged between 80 % and 101 %,with RSDs all bellow 5.9 %. LOD of aflatoxin B1,B2,G1 and G2 were0.10,0.03,0.15,0.04 μg/kg,respectively.