黄芪中毛蕊异黄酮葡萄糖苷的萃取工艺优选

Optimal Technology of Extraction Calycosin Glucoside from Astragali Radix

以超临界CO_2进行萃取,采用HPLC法测定毛蕊异黄酮葡萄糖苷的含量,优选的萃取条件为压力30 MPa,温度45℃,CO_2流量20 L/h,夹带剂为95%乙醇,加入量为药材重的5%,萃取时间2 h,粉碎粒度60目,萃取率为0.558 mg/g,结果显示超临界CO_2萃取黄芪中毛蕊异黄酮葡萄糖苷有效可行。

To investigate the technology condition of calycosin glucoside from astragali radix by supercritical CO2 fluid extraction,the content of calycosin glucoside determined by HPLC. The optimum extraction technology was as following：extraction pressure was 30 MPa, extraction temparature was 45 ℃,CO2 flow rate was 20 L/h,useing 95 % ethanol as cosolvent,cosolvent was 5 %,extraction time was 2 h,the size was 60 mesh,extracting rate of calycosin glucoside was up to 0.558 mg/g, the results showsed that supercritical CO2 fluid extraction of calycosin glucoside from astragali radix to be effective.