壳聚糖与海藻酸钙双相陶瓷骨支架的机械性能及细胞相容性

Mechanical properties and cytocompatibility of chitosan/ or calcium alginate/ biphasic ceramic bone scaffolds

背景:由羟基磷灰石和少量β-磷酸三钙构成的双相陶瓷骨具有良好的细胞相容性,但机械性能较差。目的:构建壳聚糖、海藻酸钠胶体与双相陶瓷骨复合支架材料,检测其机械性能和细胞相容性。方法:将不同浓度的壳聚糖(2%、4%、7%、10%)、海藻酸钠胶体(3%、4%、5%、7%)分别与双相陶瓷骨混合,制备壳聚糖/双相陶瓷骨支架及海藻酸钙/双相陶瓷骨支架,检测其微观形态与结构、凝固时间、抗溶解性能、抗剪切力、抗压缩力及细胞相容性。结果与结论:1凝固时间:随着胶体浓度的增大,两种复合支架的初凝时间和终凝时间均有所延长。2扫描电镜:材料均呈多孔状的微观结构,孔隙大小不一。3抗溶解性能:3%、4%、5%、7%的海藻酸钙/双相陶瓷骨支架和7%、10%的壳聚糖/双相陶瓷骨支架,在液体环境中抗溶解性能良好。4力学强度:随着胶体浓度的增大,壳聚糖/双相陶瓷骨支架的抗剪切力、抗压缩力均逐渐增强;海藻酸钙/双相陶瓷骨支架抗剪切力、抗压缩力均逐渐增强,但至7%浓度时,抗剪切力、抗压缩力均降低。5细胞相容性:壳聚糖/双相陶瓷骨支架的细胞毒性为0至1级,海藻酸钙/双相陶瓷骨支架的细胞毒性为2至3级。6结果表明,壳聚糖/双相陶瓷骨支架具备良好的机械性能及细胞相容性。

BACKGROUND： Hydroxyapatite/β-tricalcium phosphate biphasic ceramic bone has good cell compatibility, but its mechanical properties are poor. OBJECTIVE： To construct chitosan/ or calcium alginate/biphasic ceramic bone scaffolds and to detect their mechanical properties and cytocompatibility. METHODS： Different concentrations of chitosan（2%, 4%, 7%, 10%） or calcium alginate（3%, 4%, 5%, 7%） were mixed with biphasic ceramic bone to prepare chitosan/biphasic ceramic bone scaffold and calcium alginate/biphasic ceramic bone scaffold. Their morphology and structure, coagulation time, anti-dissolution properties, shear force, compressive strength and cell compatibility were detected. RESULTS AND CONCLUSION：（1） Coagulation time： with the concentration increase, the initial and final setting time of these two kinds of composite scaffolds were prolonged to some extent.（2） Scanning electron microscopy： these two kinds of composite scaffolds showed porous microstructures with different pore sizes.（3） Anti-dissolution properties： the calcium alginate/biphasic ceramic bone scaffold（3%, 4%, 5%, 7%） and chitosan/biphasic ceramic bone scaffold（7%, 10%） had good anti-dissolution properties in the liquid.（4） Mechanical strength： with the concentration increase, the shear force and compressive strength of the calcium alginate/biphasic ceramic bone scaffold were reduced.（5） Cell compatibility： the cytotoxicity of chitosan/ or calcium alginate/biphasic ceramic bone scaffolds was graded as 0-1 or 2-3, respectively. These results show that the chitosan/biphasic ceramic bone scaffold has better mechanical properties and cell compatibility than the calcium alginate/biphasic ceramic bone scaffold.