间充质干细胞条件培养液促进肺癌细胞上皮间质转化

Human bone marrow mesenchymal stem cells promote epithelial mesenchymal transition in lung cancer cells

背景:骨髓间充质干细胞与肿瘤关系的复杂性严重限制了间充质干细胞的应用,因此明确间充质干细胞在肿瘤生长与转移中的作用已成为迫切需要解决的问题。目的:探讨人骨髓来源间充质干细胞对人非小细胞肺癌A549、PAa细胞上皮间质转化的影响。方法:收集间充质干细胞上清液作为间充质干细胞条件培养液培养肺癌细胞A549和PAa,观察肺癌细胞形态、上皮间质转化标志物E-钙黏素、N-钙黏素、波形蛋白以及其转录因子Slug、Snail、Twist等的表达以及肺癌细胞运动迁移能力的变化。结果与结论:(1)与对照组对比:加入条件培养液的肺癌细胞形态发生了间质样变化,且E-钙黏素表达降低,N-钙黏素、波形蛋白mR NA与蛋白表达水平上升,转录因子Slug、Snail、Twist m RNA表达水平也明显增加,同时细胞运动迁移能力增强。(2)结果表明人骨髓来源间充质干细胞可以促进非小细胞肺癌A549、PAa细胞上皮间质转化过程,增强肺癌细胞运动迁移能力。

BACKGROUND： The complex relationship between bone marrow mesenchymal stem cells and cancers severely limit the clinical application of mesenchymal stem cells. So it is urgent to study the role of mesenchymal stem cells in tumor growth and metastasis. OBJECTIVE： To explore the effect of human bone marrow mesenchymal stem cells on epithelial mesenchymal transition in non-small cell lung cancer A549 and PAa cells. METHODS： The A549 and PAa cells were cultured with mesenchymal stem cell supernatant（mesenchymal stem cell conditioned medium, MSCs-CM）. The cellular morphology was observed under a microscope. The m RNA and protein expression of E-cadherin, N-cadherin, Vimentin, Slug, Snail, and Twist were determined by RT-PCR and western blot. Transwell and wound healing assay were used to detect the change of migration and metastatic ability. RESULTS AND CONCLUSION： Compared with the control group, the cellular morphology of experimental group showed mesenchymal-like changes. In response to MSCs-CM, there was decreased E-cadherin but increased N-cadherin, Vimentin and Slug, Snail, Twist at m RNA and protein levels compared with the control group（P 0.05）. The migration and metastatic abilities of the experimental group were also increased. So, human bone marrow mesenchymal stem cells can promote epithelial mesenchymal transition in A549 and PAa cells, and enhance the migration and metastatic abilities of A549 and PAa cells.