神经毒素6-OHDA干预后星形胶质细胞铁转运蛋白表达的变化（英文）

6-Hydroxydopamine up-regulates divalent metal transporter-1 and ferroportin-1 in C6 glioma cell lines

背景:前期的研究已经证实,在神经元和小胶质细胞中,神经毒素6-OHDA能够增加铁转入蛋白DMT1的表达,减少铁转出蛋白FPN1的表达,可能导致帕金森病黑质铁的沉积。然而,6-OHDA能否在星形胶质细胞中发挥不同的作用尚不明确。目的:观察6-OHDA作用于C6神经胶质瘤细胞(大鼠星形胶质细胞株)后,铁转运蛋白DMT1和FPN1表达变化。方法:培养大鼠星形胶质细胞C6细胞,加入10μmol/L 6-OHDA培养24 h,Western blots法检测6-OHDA干预后DMT1和FPN1蛋白表达。结果与结论:用10μmol/L 6-OHDA作用于大鼠C6星形胶质细胞24 h后,Western blots检测结果显示DMT1蛋白表达升高了2.5倍(P<0.01),FPN1蛋白表达升高了1倍(P<0.05)。提示,6-OHDA通过同时增加铁转运蛋白DMT1和FPN1表达促进铁运速率,星形胶质细胞对6-OHDA的反应与神经元和小胶质细胞不同。

BACKGROUND： Previous studies have confirmed that 6-hydroxydopamine is capable to increase the expression of divalent metal transporter-1 and reduce the expression of ferroportin-1 in the neurons and microglia, which may lead to iron deposition in the substantia nigra after Parkinson＇s disease. However, it is unclear whether 6-hydroxydopamine can play diverse roles in astrocytes. OBJECTIVE： To observe the effects of 6-hydroxydopamine on the expression of divalent metal transporter-1 and ferroportin-1 in rat C6 glioma cell lines. METHODS： C6 glioma cell lines from rats were cultured in 10 μmol/L 6-hydroxydopamine for 24 hours. Then, protein expressions of divalent metal transporter-1 and ferroportiner-1 were measured by western blot method. RESULTS AND CONCLUSION： The protein expressions of divalent metal transporter-1 and ferroportin-1 in C6 glioma cell lines were increased by 2.5 times（P 0.01） and 1 time（P 0.05）, respectively, after treatment with 6-hydroxydopamine. These findings indicate that 6-hydroxydopamine can promote iron transport rate in astrocytes by increasing both divalent metal transporter-1 and ferroportin-1 expressions, and astrocytes has a different response to 6-hydroxydopamine from neurons and microglia.