瘀胆血清诱导骨髓间充质干细胞向肝细胞的分化

Effect of cholestatic serum on differentiation of bone marrow mesenchymal stem cells into hepatocytes

背景:在一定的条件下,骨髓间充质干细胞可发生分化,是肝细胞的重要来源之一,多种因子会对其产生诱导作用。目的:探讨瘀胆血清在骨髓间充质干细胞向肝细胞分化中的诱导作用。方法:制备瘀胆动物模型,获得瘀胆血清。将分离的大鼠骨髓间充质干细胞分为3组,分别利用无血清肝细胞培养液、无血清肝细胞培养液+瘀胆血清、无血清肝细胞培养液+正常血清进行培养。结果与结论:无血清肝细胞培养液+瘀胆血清组的甲胎蛋白阳性表达率、角蛋白18阳性表达率和白蛋白质量浓度均显著高于无血清肝细胞培养液组和无血清肝细胞培养液+正常血清组(P<0.05)。表明瘀胆血清具有一定的诱导作用,可以诱导骨髓间充质干细胞向肝细胞进行分化。

BACKGROUND： Under certain conditions, bone marrow mesenchymal stem cells can be differentiated into hepatocytes, which are an important source of liver cells. Moreover, multiple factors can be involved in this induced differentiation process. OBJECTIVE： To investigate the inducible effect of cholestatic serum on the differentiation of bone marrow mesenchymal stem cells into hepatocytes. METHODS： Cholestatic animal model was prepared in rats to extract cholestatic serum. Bone marrow mesenchymal stem cells isolated from rats were divided into three groups and cultured in serum-free hepatocyte medium, serum-free hepatocyte medium plus cholestatic serum, serum-free hepatocyte medium plus normal serum, respectively. RESULTS AND CONCLUSION： The positive expression of alpha fetoprotein and keratin 18 and mass concentration of albumin were significantly higher in the serum-free hepatocyte medium plus cholestatic serum group than the other two groups（P〈0.05）. These findings indicate that cholestatic serum has a certain inducible role in the differentiation of bone marrow mesenchymal stem cells into hepatocytes.