三七疫霉病拮抗根际土壤细菌的筛选及初步鉴定

Screening and Preliminarily Identification of Rhizosphere Soil bacterial Controlling Phytophthora Cactorum of Panax Notoginsing

为了筛选对环境友好的生防细菌防治三七疫霉病,通过高温处理、稀释涂布法分别从三七健康及患病植株根际土壤分离了21和16株可培养细菌,并以对峙培养法分别筛选到6和7株拮抗菌株,上述13株拮抗菌株经生理生化特征鉴定为芽孢杆菌属。结果表明,健康植株根际土壤有较丰富的可培养细菌,而患病植株根际土壤可培养细菌中的拮抗菌株较丰富,表明健康植株与患病植株根际土壤的拮抗细菌丰度发生了变化。解淀粉芽孢杆菌B1的抑菌带宽达8.5mm,具有一定的潜在生防价值。

To screen eco-friendly bio-control bacteria controlling Phytophthora cactorum of Panax notoginsing, 21 and 16 strains rhizobacteria were separated respectively from healthy and infected Panax notoginsing by thermal processing, tissue crashing method, dilution coating method, additionally 6 and 7 strains antagonistic bacteria among them were respectively selected by confrontation culture method, then total of antagonistic bacteria above were identified as Bacillium spp. through physiological and biochemical characteristics. The results suggested that there were richer cultural bacteria in rhizosphere soil of healthy plants, in contrast that there were relatively richer antagonistic bacteria in rhizosphere soil of infected plants. In another word, the richness of antagonistic bacteria changed in rhizosphere soil of healthy and infected plants. Bacillus amyloliquefaciens B1 identified by physiological and biochemical characteristics, which had a certain potential bio-control value because of inhibition zone width up to 8.5 mm.