肝素抗凝血浆与血清中微量元素测定的对比分析

Comparative Analysis of the Determination of Trace Elements in the Plasma and Serum of Heparin

目的研究肝素抗凝血浆代替血清进行微量元素测定的可行性。方法选择2014年6月在汉川市人民医院接受生化检验的272例患者,采用Beckman DXC800型全自动生化分析仪,同时检测患者的肝素抗血浆和血清中锌(Zn)、铁(Fe)、铜(Cu)3种微量元素的浓度。结果肝素抗凝血浆锌、铁与血清中的锌、铁离子浓度比较无显著差异,差异无统计学意义(P>0.05);血浆中铜离子的浓度明显低于血清中铜离子的检测值,差异有统计学意义(P<0.05),但通过回归方程可以校正。结论肝素抗凝检测方法能够有效避开血液的凝固过程,在短时间内分离标本并检测,从而减少放置误差。但肝素抗凝血浆铜的检验还应进行回归校正。

Objective To study the feasibility of heparin anticoagulant plasma instead of serum for thedetermination of trace elements. Methods 272 patients who were accepted biochemical test in ourhospital from June, 2014. and they were treated by Beckman DXC800 automatic biochemical analyzer.Then, the concentration of heparin in plasma and a variety of serum trace elements in patients weresimultaneous detected. Results There was no significant difference in the concentration of zinc and ironin the plasma and serum of heparin, and the difference had not statistically significant （ P 〉 0. 05 ） ; Inaddition, the concentration of copper ion in plasma was effectively lower than that in serum; Throughregression analysis, the low levels of copper in the plasma may be caused by the release of blood duringthe coagulation of platelets, the difference had statistically significant （ P 〈 0. 05 ） . Conclusion Themethod of heparin anticoagulation can effectively avoid the coagulation of blood, and can be used toseparate the samples in a short time, so as to reduce the dissolution and reduce the error. However, the test of heparin anticoagulant plasma copper should be carried out by regression.