灭蚊真菌贵阳腐霉对蛋白核小球藻的安全性检测

A Test on the Safety of Pythium guiyangense to Chlorella vulgaris

目的:检测贵阳腐霉对蛋白核小球藻的安全性。方法:用小球藻培养液培养小球藻,实验组在培养液中加入贵阳腐霉,对照组不加贵阳腐霉;观察并比较培养6 d时各组培养液的颜色和色深,在100倍显微镜下观察各组小球藻细胞形态;比较培养3 d和6 d时各组小球藻细胞密度,评估贵阳腐霉对蛋白核小球藻的安全性。结果:培养6 d时,各组培养液都显示出鲜艳的绿色,无明显区别;在100倍显微镜下观察各组小球藻细胞形态也正常,各组间无明显差别;培养3 d和6 d时各组的小球藻细胞密度,差异无统计学意义(P>0.05)。结论:贵阳腐霉对蛋白核小球藻具有较高的安全性。

Objective： To test the safety of Pythium guiyangense to Chlorella vulgaris. Methods： C.vulgaris was cultured in liquid medium,and was divided into experimental group and control groups.In experimental group,mycelia of P. guiyangense was added,while no fungus was added in control groups. During incubation,the condition of whole culturing liquid and cellular morphology of C. vulgaris cells were observed under the100 times microscope. C. vulgaris cell density was tested with densityometry,and the results were compared between the two groups at day three and day six. Finally,the safety of Pythium guiyangense to Chlorella vulgaris was evaluated. Results： 6 days after culture,culturing liquid of both groups showed bright green color and no significant difference was found between groups. The cellular morphology of C. vulgaris cells under the100 times microscope was normal in both groups and no significant difference was found. In terms of cell density of C. vulgaris,there was no statistical difference in both groups between 3 day culture and 6 day culture. Conclusion： P.guiyangense is safe to C. vulgaris.