冬虫夏草中甘露醇和虫草素的TOF-SIMS分析

TOF-SIMS Study of Mannitol and Cordycepin in Cordyceps Sinensis

用飞行时间二次离子质谱(TOF-SIMS)分析研究了冬虫夏草中两个最重要的药效活性物质,甘露醇和虫草素,重点研究对应质量数251amu的质谱峰含有的化学信息。利用TOF-SIMS高质量分辨率的优势,检测并识别251和252amu质谱峰的信号有可能不是保健药理活性物质虫草素C_(10)H_(13)N_5O_3(251amu)产生的分子离子峰M^+,[M+H]^+,这或许就是文献中有关研究虫草素存在争论的原因。TOF-SIMS分析结果为进一步解读251amu质谱峰的化学内含、深入研究冬虫夏草中虫草素提供了依据;同时就甘露醇对应的(181amu附近)TOF-SIMS负离子质谱峰做了细致的解读,确认181amu质谱峰是识别冬虫夏草中甘露醇的可靠依据。本研究证明,TOF-SIMS是分析、研究、鉴别冬虫夏草的有效手段。

Cordyceps sinensis is a well-known traditional Chinese medicine;it is also called DongChongXiaCao（winter worm summer grass）in Chinese.Mannitol and cordycepin,the most important two pharmacological active components of cordyceps sinensis,were studied with TOF-SIMS.This Study was focused on the chemical information including 251 amu mass peak.Based on high mass resolution of TOF-SIMS analysis,the fragment ions of 251 and 252amu detected in Cordyceps sinensis may not be the molecular ion M~＋and/or[M＋H]~＋of cordycepin,which ispossiblely the root cause of the argument in the study of cordycepin in published papers.It could be a basis for further study of cordycepin components of cordyceps sinensis in the future.The 181 amu mass peak of minus ion in mannitol was also studied in detail and was certified to be a reliable evidence of mannitol.This research shows that TOF-SIMS has been proven as an effective method in the study of cordyceps sinensis.