原发性乳腺癌AS160分子磷酸化水平与肿瘤细胞增殖活性指标的相关性分析

Correlation of AS160 phosphorylation with tumor cell proliferative activity in primary breast cancer

目的探讨乳腺癌组织中磷酸化AS160(Thr642)水平上调与肿瘤细胞增殖活性指标之间的关系。方法回顾性分析中国人民解放军第八五医院2003年1月至2015年1月收治的152例乳腺癌患者的临床病理资料。采用免疫组织化学方法检测肿瘤组织中磷酸化AS160(Thr642)及增殖指标Ki-67(MIB-1)水平,对肿瘤组织作核分裂象计数,并观察乳腺癌细胞株MDA-MB-231中磷酸化AS160(Thr642)的表达特点。采用Spearman检验分析磷酸化AS160(Thr642)与核分裂象计数、Ki-67(MIB-1)的相关性。结果磷酸化AS160(Thr642)与核分裂象计数、Ki-67(MIB-1)呈显著正相关(r=0.493、0.472,P均<0.001)。乳腺癌组织与细胞株中的有丝分裂象细胞磷酸化AS160(Thr642)呈强阳性。结论磷酸化AS160分子的表达能反映乳腺癌细胞的增殖活性,是乳腺癌的1个有价值的标志物和治疗靶分子。

Objective To investigate the correlation of phosphorylation AS160（Thr642） regulation with tumor cell proliferative activity in breast cancer tissue. Methods The clinical data of 152 patients with breast cancer in 85 Hospital of People＇s Liberation Army from January 2003 to January 2015 were analyzed retrospectively. Immunohistochemical method was used to determine phosphorylation AS160（Thr642） and proliferation Ki-67（MIB-1） levels. Mitotic index was evaluated,and the expression characteristics of phosphorylation AS160（Thr642）was investigated in breast cancer cell line MDA-MB-231. The correlations of phosphorylation AS160（Thr642） with mitotic index and Ki-67（MIB-1） were analyzed by Spearman nonparametric test. Results The phosphorylation AS160（Thr642） was positively correlated with mitotic index（r=0.493,P〈0.001） and Ki-67（MIB-1）（r=0.472,P〈0.001）. The phosphorylation AS160（Thr642） in mitotic tumor cells was strongly positive in cell line and breast cancer tissue. Conclusions The phosphorylation AS160 is correlated with tumor cell proliferative activity and might be useful as a marker and a potential treatment target for breast cancer.