牛磺酸对严重腹腔感染大鼠肠黏膜屏障功能的影响

Effect of taurine on intestinal mucosal barrier function in rats with severe abdominal infection

目的通过建立大鼠严重腹腔感染模型,检测血浆二胺氧化酶（diamine oxidase,DAO）的活性及D-乳酸含量,探讨牛磺酸对严重腹腔感染大鼠肠黏膜屏障的保护作用。方法实验于2015年5～7月将54只雄性SD大鼠随机分为三组：对照组（C组）、腹腔感染组（I组）和治疗组（T组）,每组18只。C组仅轻微翻动盲肠后关腹,I、T两组采用盲肠结扎穿孔法（cecal ligation plus puncture,CLP）。T组于术后即刻腹腔注射牛磺酸溶液（200 mg/kg）,而C组、I组术后即刻腹腔注射等体积生理盐水。各组分别于造模后6、12、24 h随机取6只大鼠,检测血浆DAO活性和D-乳酸含量。结果 C组各检测指标在各时间点无明显差异,差异无统计学意义（P〉0.05）,I组、T组各检测指标均随时间延长呈增高趋势。I组、T组各检测指标在各时间点均高于C组,差异均有统计学意义（P〈0.05）,T组各检测指标在各时间点均低于I组,差异均有统计学意义（P〈0.05）。结论牛磺酸对大鼠严重腹腔感染时肠黏膜屏障功能有一定的保护作用。

Objective To explore the possible protective effect of taurine on intestinal mucosal barrier function in rats with severe abdominal infection through established the model of rats with severe abdominal infection, measuring the activity of diamine oxidase（DAO） and the concentration of D-lactic acid in plasma. Methods From May to July 2015fifty-four SD males rats were randomly divided into three groups, 18 rats in each group）： control group（group C）, abdominal infection group（group I） and treatment group（group T）. The rats of group C were only slightly changed the cecal several times before definitive abdominal closure. The rats in group I and group T were established by cecal ligation and puncture（CLP）. The rats in group T received intraperitoneal injection of taurine（200 mg/kg） immediately after the operation, however, the rats in group C and group I received intraperitoneal injection of equivoluminal normal saline.Six rats in each group were randomly picked up at 6, 12, 24 h after operation to have the activity of diamine oxidase（DAO） and the concentration of D-lactic acid in plasma measured. Results There was no significant difference at each time point of each detection index in group C（P〈0.05）. In group I and group T, each detection index was rising with increased postoperative time, and they were higher than those of group C at each time point, there was significant difference（P〈0.05）. Compared with group I, each detection index of group T was lower at each time point, there was significant difference（P〈0.05）. Conclusion Taurine has a certain protective effect on intestinal mucosal barrier function in rats with severe abdominal infection.