夹带剂在超临界CO_2萃取黄芪甲苷中的影响

Effects of Entrainer on Astragaloside Extraction from Astragalus by Supercritical CO_2

目的:研究夹带剂对超临界CO_2萃取黄芪中黄芪甲苷的影响。方法:以夹带剂加入方式、夹带剂种类、夹带剂加入量及夹带剂流速为考察因素,黄芪甲苷的提取率作为考察指标,用L_9(3~4)正交表优化工艺条件,并用高效液相色谱法对黄芪甲苷进行含量测定。结果:夹带方式选择预浸+动态萃取模式,以95%乙醇作为夹带剂,加入量为300 ml,加入流速10 ml·min^(-1);在以下条件进行超临界萃取时,黄芪甲苷提取率可达到0.040 7%:萃取温度45℃,出口温度55℃,萃取压力35 MPa,95%乙醇液态收集萃取物,动态萃取时间2 h。结论:本实验优选的工艺对超临界CO_2萃取黄芪中黄芪甲苷的提取有着明显提高。

Objective： To research the effects of entrainer on extracting astragaloside from astragalus by supercritical CO_2. Methods： The optimum extraction technology conditions were investigated by an L_9（ 3～4） orthogonal experiment with the extraction rate of astragaloside as the evaluation index and the addition way,variety,amount and flow rate of the entrainers as the investigation factors. The content of astragaloside was determined by HPLC. Results： The addition way of entrainer was the presoak treatment combined with the dynamic extraction mode,and the entrainer was 95 % alcohol with 300 ml of infusing volume and 10 ml·min^（-1）flowing volume. The best factors of supercritical fluid extraction were as follows： the extraction pressure was 35 MPa,the extraction temperature was 45℃,the outlet temperature was 55℃,the extracts were collected with 95 % alcohol,and dynamic extraction time was 2 h. Under the above process conditions,the extraction rate of astragaloside was 0.040 7%. Conclusion： The extraction of astragaloside from astragalus by supercritical CO_2 extraction with the optimized process is significantly improved.