狗肝菜多糖P2B对CCl_4致L-02肝细胞损伤的保护作用

Protective Effect of Diclipterachinensis Polysaccharide P2B on Carbon Tetrachloride-induced Injury of Liver Cell Line L-02

目的:探讨狗肝菜多糖(P2B)对四氯化碳(CCl_4)诱导的肝L-02细胞损伤的保护作用。方法:培养人肝L-02细胞,建立CCl_4肝细胞损伤模型,分组实验:正常对照组、CCl_4损伤组和不同浓度的P2B(0.125、0.25和0.5 mg·ml^(-1))样品组。采用MTT法检测生化分析培养液中丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)以及细胞内超氧化物歧化酶(SOD)、丙二醛(MDA)含量。结果:与CCl_4损伤组相比,各P2B样品组细胞活力明显增强,上清液中ALT、AST活性明显降低;细胞内SOD明显升高、MDA含量降低。结论:P2B对CCl_4诱导的体外L-02肝细胞损伤均具有保护作用,随着浓度的增加,作用也随着增强,其机制可能与其抗氧化作用有关。

Objective： To evaluate the protective effect of Diclipterachinensis polysaccharide P2B on liver cell line L-02 injury induced by carbon tetrachloride（ CCl_4）. Methods： The human liver L-02 cells were cultured,and the injury model was built by CCl_4.The L-02 cells were divided into the normal control group,the CCl_4-damaged group,and the P2B sample groups（ 0.125,0.250 and0.500 mg·ml^（-1））. The contents of alanine aminotransferase（ ALT）,aspartate aminotransferase（ AST）,superoxide dismutase（ SOD）and malondialdehyde（ MDA） were determined by MTT assay. Results： Compared with the CCl_4-damaged group,P2B could improve the activity of L-02 cells,and the activity of AST and ALT in the supernatant was significantly reduced,and the content of SOD in the cells was increased and that of MDA was decreased. Conclusion： P2B can significantly prevent L-02 cells from the damage induced by CCl_4 in a dose-dependent manner,and the mechanism may be related with the anti-oxidative activity of P2B.