HPLC法测定四叶参中四叶参皂苷的含量

Determination of Codonoposide in Codonopsis Lanceolata Benth.et Hook.F.by HPLC

目的:建立HPLC法测定四叶参中四叶参皂苷的含量测定方法。方法:色谱柱为Kromasil C_(18)(250 mm×4.6 mm,5μm);流动相为0.1%磷酸水-乙腈(70∶30);流速为1.0 ml·min^(-1);柱温为30℃;检测波长为203 nm。结果:四叶参中四叶参皂苷与其他组分分离良好,线性范围为1.09~17.41μg(r=0.999 9),加样回收率为98.46%(RSD=1.64%,n=6)。结论:该方法准确性、重复性好,可用于四叶参中四叶参皂苷的含量测定。

Objective： To establish an HPLC method for the determination of codonoposide in Codonopsis lanceolata Benth. et Hook.F..Methods： The HPLC analysis was performed on a Kromasil C_（18）column（ 250 mm × 4.6 mm,5 μm）. The mobile phase was0.1% phosphoric acid- acetonitrile（ 70 ∶ 30） and the flow rate was 1.0 ml·min^（-1）. The detection wavelength was 203 nm and the column temperature was 30 ℃. Results： Codonoposide in Codonopsis lanceolata Benth. et Hook. F. had a good separation from the other components,and a good linear relationship was obtained within the range of 1.09-17.41 μg（ r = 0.999 9）. The average recovery was98.46%（ RSD = 1.64%,n = 6）. Conclusion： The method is practicable with promising repeatability,which can be applied in the content determination of codonoposide in Codonopsis lanceolata Benth.et Hook.F..