可溶性PD-1和调节性T细胞在肺炎支原体感染和哮喘的实验研究

sPD-1 and Regulatory T Cells in Asthma and Mycoplasma Pneumoniae Infection

目的:探讨可溶性PD-1(soluble PD-1,s PD-1)和调节性T细胞(Regulatory T lymphocyte,Treg)在肺炎支原体(mycoplasma pneumoniae,MP)感染与哮喘模型小鼠关系中的作用。方法:20只BALB/c小鼠随机分为4组(n=5),其中2组建立鸡卵白蛋白(ovalbumin,OVA)致敏哮喘模型,将MP菌液滴入其中1组OVA小鼠和1组对照小鼠,形成OVA致敏并MP感染组,OVA致敏组,MP感染组及正常对照组。采用酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)方法分别检测各组小鼠血清、肺泡灌洗液(Bronchoalveolar Lavage Fluid,BALF)中s PD-1的浓度;采用流式细胞术检测脾脏组织中Treg细胞的比例。结果:1与正常对照组相比,OVA致敏并感染MP组、单纯OVA致敏组和单纯MP感染组小鼠血清s PD-1的浓度均升高,差异有统计学意义(P<0.05),BALF中的s PD-1的浓度亦增高,但无统计学差异;2与正常对照组相比,OVA致敏并感染MP组、单纯OVA致敏组小鼠的脾脏Treg细胞占淋巴细胞的比例下降,而单纯MP感染组Treg细胞的比例升高,差异具有统计学意义(P<0.05)。结论:s PD-1和Treg细胞可能参与了哮喘小鼠感染MP的发病。

Objective： Discussion whether s PD-1（soluble PD-1）and Treg cells（regulatory T cells） be involved allergic model and Mycoplasma pneumoniae infection. Methods： 20 BALB/c mice were randomly divided into 4 groups, two groups of which were sensitized and challenged with OVA. One of OVA mice groups and one of control groups were inoculated intranasally with MP. We detected the concentration of s PD-1 in serum and bronchoalveolar lavage with Enzyme-linked immunosorbent assay. The proportion of regulatory T cells in the spleen was detected by flow cytometry. Results： 1Compared with the control group,the concentration of s PD-1 in serum and bronchoalveolar lavage in other goups were increased, and the concentration of s PD-1 in serum was statistically significant（P0.05）.2Compared with normal mice, the proportion of regulatory T cells in the spleen of groups which estabilished allergic airway and estabilished allergic airway with MP infectin were decreased, the difference between groups was statistically significant（P 0.05）. Conclusion：Elevated s PD-1 and reduction of Treg may be involved in the relation between asthma and Mycoplasma pneumoniae infection.