人工栽培桑黄不同生长期主成分含量的动态变化

Study on dynamic changes in the main components of Phellinus igniarius during different growing periods

目的:研究人工栽培桑黄不同生长期主要成分含量的变化,确定最佳采收期。方法:利用分光光度仪,采用硫酸蒽酮法测定总多糖含量;HPLC法同时测定Inoscavin A和Hypholomine B的含量,通过多指标含量测定比较不同样品的成分含量。结果:不同生长期的桑黄主要成分的含量变化趋势不同,其中总多糖与Inoscavin A的含量均以5月12日(出菇22d)最高,分别为0.74%和0.16mg/g;而Hypholomine B的含量以6月12日(出菇53d)最高,为0.59mg/g;亩产量以7月3日(出菇74d)最高,为0.35t。结论:人工栽培桑黄不同生长期的含量有一定的差异,主成分含量监测引导下的采收期确定,将有利于桑黄的质量控制与开发利用。

Objective： To study dynamic changes in the main components of Phellinus igniarius during different growing periods, and to determine the optimal harvest time. Methods： The content of total polysaccharides was measured on the spectrophotometer by the anthrone sulfuric acid method. The contents of Inoscavin A and Hypholomine B were measured by high performance liquid chromatography（HPLC）. The contents of main components were compared among the different Phellinus igniarius by the dectection of multi-index. Results： The main componenets of Phellinus igniarius domenstrated various regularity during different growth period. Both of the contents of total polysaccharides and Inoscavin A came into the highest in May 12th（fruiting 22d）, up to 0.74% and 0.16mg/g, respectively. The content of Hypholomine B came into the highest in June 12th（fruiting 53d）, up to 0.59mg/g. The acre yield came into the highest in July 3rd（fruiting 74d）, up to 0.35 t, Conclusion： The Phellinus igniarius during different growing periods contained different components. The active ingredients monitor would help to guide an optimal harvest time for the Phellinus igniarius, resulting in a better quality control and utilization of Phellinus igniarius.