HPLC内标手性拆分法分析保健食品雨生红球藻片中虾青素光学异构体

Chiral Separation and Analysis of Astaxanthin Stereoisomers in Health Food Yushenghongqiuzao Tablets by HPLC Internal Standard Method

目的建立HPLC内标手性拆分法分析雨生红球藻片中2种虾青素光学异构体含量的方法。方法采用HPLC法,以反式-β-8＇-胡萝卜醛为内标,色谱柱：YMC-Carotenoid TMS（250 mm×4.6 mm,5μm）;流动相：甲醇-甲基叔丁醚-1%磷酸溶液,梯度洗脱;流速：1.0 ml·min-1;检测波长：474 nm。结果虾青素异构体在0.5185～5.185μg·ml-1范围内进样量与峰面积线性关系良好（r=0.9999）。平均回收率为99.40%,RSD为4.53%。结论方法简便、快速准确、灵敏度高,重复性好。

Objective To establish a method for chiral separation and determination of astaxanthin stereoisomers in Yushenghongqiuzao tablets using HPLC internal standard method. Methods With trans-β-Apo-8＇-carotenal as the internal standard,the HPLC system was performed on a YMC-Carotenoid TMS column（ 250 mm × 4. 6mm,5 μm） with methanol-methyl tert-butyl ether（ MTB）-1 % phosphoric acid as the mobile phase in a gradient mode at a flow rate of 1. 0 ml·min- 1,detected with UV at 474 nm. Results There was a good linear relationship within the rage 0. 5185- 5. 185 μg·ml- 1of astaxanthin stereoisomers,and the correlation coefficient（ r） was0. 9999. The result（ n = 6） of the average recovery was 99. 40 %（ RSD = 4. 53%）. Conclusion This method is simple,quick,accurate and sensitive.