氯胺酮对SD幼鼠海马GAP-43蛋白表达的影响

Effect of Ketamine on GAP-43 Protein Expression in Hippocampus of SD Rats

目的：分析氯胺酮对SD幼鼠海马GAP-43蛋白表达的影响。方法：取7~10日龄的SD幼鼠36只,随机分为A、B、C三组各12只。A组注射生理盐水50 mg/kg,B组注射氯胺酮25 mg/kg,C组注射氯胺酮50 mg/kg。24 h后处死小鼠取海马组织,分别采用免疫组化与Western Blotting检测海马GAP-43蛋白的表达情况。结果：经图像分析软件处理后得知,A组、B组、C组条带灰度值分别为187.43±7.98、164.87±8.35、154.31±6.98,组间比较差异均有统计学意义（P〈0.01）。A组、B组、C组GAP-43阳性细胞计数分别为（87.45±14.98）个/mm2、（66.48±12.36）个/mm2、（35.32±9.93）个/mm2,组间比较差异均有统计学意义（P〈0.01）。结论：氯胺酮可明显降低SD幼鼠海马GAP-43蛋白的表达水平,这可能与其影响幼鼠神经系统发育有关。

Objective： To study the effect of ketamine on the expression of GAP-43 protein in hippocampus of SD rats. Methods：Thirty six SD rats who were 7 to 10 days old,were randomly divided into three groups. Group A of 12 rats were injected with saline50 mg / kg,group B of 12 rats were injected with ketamine 25 mg / kg,group C of 12 rats were injected with ketamine 50 mg / kg. All rats were sacrificed and the hippocampuses were drawn out after 24 hours. Immunohistochemistry and Western Blotting were used to detect the expressions of hippocampal GAP-43 protein. Results： Then we analyzed the results by image analysis software,gray stripe value of group A was 187.43±7. 98,striped gray value of group B was 164. 87 ± 8. 35,striped gray value of group C was 154. 31 ± 6. 98,there were statistical differences between three groups（ P〈0.01）. The GAP-43 positive cell count in group A was（ 87.45± 14.98） / mm2,group B was（ 66.48± 12. 36） / mm2,group C was（ 35.32± 9.93） /mm2,there were statistical differences between three groups（ P 0. 01）.Conclusion： Ketamine can significantly reduce the expression level of GAP-43 protein in the hippocampus of SD rats,which may be related to the development of the nervous system in young rats.