白细胞介素-4和白细胞介素-13基因多态性对哮喘患儿IgE表达的影响

The Influence of IL-4 and IL-13 Gene Polymorphism on Ig E Level in Children with Asthma

目的:探讨白细胞介素-4(IL-4)和白细胞介素-13(IL-13)基因多态性对哮喘患儿Ig E表达的影响。方法:采用聚合酶链式反应(PCR)检测173例哮喘患儿及56例健康儿童的IL-4和IL-13基因限制性长度片段,及不同基因型患儿Ig E的表达水平。结果:哮喘患儿IL-4基因-589位点基因多态性和等位基因与健康对照儿童比较差异无统计学意义(P>0.05)。哮喘患儿IL-13基因+1923位点基因多态性和等位基因与健康对照儿童比较差异有统计学意义(P<0.01)。IL-4基因-589和IL-13基因+1923位点T等位基因患儿Ig E水平显著高于C等位基因患儿(P<0.01)。结论:哮喘患儿IL-13基因多态性与健康儿童不同,IL-4基因-589位点和IL-13基因+1923T等位基因是导致哮喘患儿Ig E水平升高的可能原因。

Objective： To study the influence of interleukin-4（ IL-4） and interleukin-13（ IL-13） gene polymorphism on Ig E level in asthma children. Methods： PCR was applied to detect the restriction fragment length of IL-4 gene and IL-13 gene in 173 asthma children and 56 healthy children and the Ig E expression levels of different genotypes. Results： The gene polymorphism and alleles of IL-4-589 had no significant difference between two control（ P〉0.05）. The gene polymorphism and alleles of IL-13＋1923 in asthma children were significantly different between two groups（ P〈0.01）. The Ig E level in asthma children with T allele of IL-13＋1923 gene and IL-4-589 were significantly higher than those in C allele（ P〈0.01）. Conclusion： The IL-13 gene polymorphism was different from healthy control and the T allele of IL-4-589 and IL-13＋1923 may play an important role on the increase of Ig E in asthma children.