长寿人群CETP I405V基因多态性与血脂水平的相关性

Association of CETP I405V polymorphism with serum lipid level in a long-lived population

目的探讨广西红水河流域长寿群体的胆固醇酯转移蛋白(CETP)I405V基因多态性与血脂的关系。方法研究对象为523例广西红水河流域长寿人群(HL),对照组为498例同地区非长寿人群(HNL),用PCR-RFLP方法进行CETP I405V基因分型,并分析其多态性对血脂的影响。结果 1)该地区人群的等位基因以Ⅰ(58.96%)为主,基因型以Ⅱ(34.28%)、Ⅳ(49.36%)为主。性别分层和BMI分层后,HL组的基因型及等位基因频率的分布均未发现明显差异;HNL组女性Ⅱ、ⅤⅤ基因型频率高于男性,Ⅳ则低于男性(P<0.01),HNL超重亚组ⅤⅤ、Ⅱ的频率高于正常体重亚组,Ⅳ反之(P<0.05)。2)不同基因型对血脂无显著影响。因组别不同、性别分层、BMI分层,血脂水平在不同基因型均出现不同变化趋势,但乏规律可循。结论 CETP I405V多态性对广西红水河流域长寿人群的血脂水平影响较小,其血脂格局可能源自其他未知遗传变异及其与环境的交互作用。

Objective To explore the polymorphism of CETP I405 V in a long-life span population residing Guangxi Hongshuihe River Basin and explore the possible association with lipid level and long longevity. Methods Genotyping of CETP I405 V was performed with PCR-RFLP for 523 long-lived individuals（ HL） and 498 non-long-lived individuals（ HNL） from Guangxi Hongshuihe River Basin. Association analyses were conducted among genotypes and serum lipids. Results 1） The main allelic（ Ⅰ） and genotypic（ Ⅱ,Ⅳ） frequencies of the people in this area were 58. 96%,34. 28%,49. 36%. When stratified by gender and BMI,no significantly different allelic and genotypic frequency on CETP I405 V loci was found in the HL. However,when stratified by gender,females in HNL were found to carry more Ⅱ,ⅤⅤ and less Ⅳ than did males（ P〈0. 01）. The frequency of ⅤⅤ and Ⅱ in the HNL was found higher while Ⅳ was lower in the overweight subgroup than that in the non-overweight（ P〈0. 05）.2） No difference was revealed among genotypes in both HL and HNL of serum lipid levels. When stratified by group,gender and BMI,serum lipid levels among the genotypes presented different changing trends,but lack of rules to follow. Conclusions There is no correlation between the polymorphism of CETP I405 V and natural longevityof in Guangxi Hongshuihe region. The lipid profiles of long-lived individuals might be modulated by other unrevealed variants and their interplay with non-genetic factors.